The Influence Of Swine Source Lactobacillus Plantarum On The Antioxidant Capacity And Immunity In Piglets

The growth characteristics, resistance, and preparation process of a Lactobacillus plantarum and its effect on producting performance has been studied in this experiment. The results as follows:(1) The optimum growth temperature and growth pH value of this Lactobacillus plantarum was 30℃and 6.0, respectively. Lactose and soy peptone were the optimum carbon source and nitrogen source. The optimum buffer salt matching of this strain was anhydro-sodium acetate 3g/L+ anhydro-dipotassium phosphate 2g/L. The cell density could achieve 1010cfu/mL in the natural state. With secondary liquid culture, The number of viable bacteria could increase at second culture with equal volume, but no log increase.(2) The Lactobacillus plantarum could grow in culture medium of MRS contain 0.1% pigs bile salt culture. The survival rate of this strain was up to 58.8% when incubated in artificial gastric juice at pH 3.0 for 2 h. while the survival rate was only 0.1‰when incubated at pH 2.0 for 2 h. Artificial intestinal juice had inhibition to this strain. The survival rate of this strain was 90% when sustained at 55℃for 60 s, but was only 6.3% at 60℃for 10 s.(3) The supernatant and the bacterium liquid of this Lactobacillus plantarum had inhibition to Escherichia coli, Salmonella choleraesuis, and Staphylococcus aureus. The extracellular polysaccharide was 748.46mg/L when cultured in MRS medium at pH 6.0, 30℃for 48 h. The degradation of cholesterol was 53% at 12 h.(4) The Lactobacillus plantarum had strong resistance to hydrogen peroxide and hydroxyl radical scavenging activity. The activity of this strain was decreased 50% when cultured in 1.0mmol/L hydrogen peroxide for 4 h. The hydroxyl radical scavenging rate was 23.57% of 109cfu/mL cell suspension. The scavenging rate for superoxide anion was 39.22% of the cell disruption extract, and clearance rate for DPPH was 13.39%.(5) The microcapsule of Lactobacillus plantarum had high acid resistance, enteric, and storage stability when using 3% sucrose, 5% lactose, 5% skim milk, and 5% dextrin as freeze-drying protective, 10% talc as supplementary materials, 1.5% sodium alginate as wall material, and fixed in 2% calcium chloride for 20 m.(6) The Lactobacillus plantarum could effectively promote the growth, improve the feed efficiency, reduce the mortality rate of piglets, decrease the of content of serum MDA and Hp, increase the serum IL-2 levels and the activity of SOD and GSH-Px when added alone or combined with yeast fungus. Although combined this strain with Bacillus could increase the feed efficiency, reduce the mortality rate of pigs, decrease the content of serum MDA and Hp, and improve the serum IL-2 levels and the activity of SOD, there was no positive effect on the activity of serum GSH-Px and the growth of pigs. No immune synergy to blue ear disease antibody was observed when adding this strain alone or combined with yeast fungus or bacillus.