| Post-transcriptional gene silencing (PTGS) is a kind of RNA silencing in plant, which the plant will initiate the gene silencing of virus genes when infected by virus. Through the PTGS mechanism in plant, virus derived recombinant vector carrying target gene can be used to induce plant endogenous gene silencing, so called virus-induced gene silencing (VIGS).In this thesis, we set up the VIGS system with tobacco rattle virus (TRV) derived vector in our laboratory. Using this TRV derived vecotor, we successfully silenced the VIGS reporter gene–phytoene desaturase (PDS) gene, which result in photobleaching phenotype. The VIGS system will benefit the large scale gene function analysis in tomato. In addition, we also optimized the tomato growth conditions after inoculated with VIGS vector in order to get good VIGS effectiveness.Tomato powdery mildew is a worldwide fungal disease caused by Oidium neolycopersici, which can grow through the whole growth period. Tomato powdery mildew can significantly decrease tomato production level. The prevention and control of tomato powdery mildew spreading is fungicide, which increases production cost and cause environmental pollution. Using transgenic method to obtain resistance tomato against O. neolycopersici is one of the best choices to control tomato powdery mildew. Therefore, identifying the key genes of tomato resistance response against O. neolycopersici becomes very important.We previously identified a candidate key gene (DL-II-C1) of tomato resistance response against O. nelolycopersici using cDNA-AFLP technology and in-silico mapping. In this thesis, functional analysis of this candidate gene was carried out by using VIGS system. The recombinant vector carring DL-II-C1 was inoculated on the wild type tomato G1.1560, which is resistant against O. neolycopersici. The results showed G1.1560 tomato lose its resistance against O. neolycopersici, indicating that DL-II-C1 is a key gene of tomato powdery resistance response. |
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