ISSR Variation Among Different Populations Of Coelomactra Antiquate And Pseudosciaena Crocea Richardson

The Inter Simple Sequence Repeat (ISSR) technique was applied to assess the genetic variation and the genetic differentiation among three populations of Coelomactra antiquate, and Pseudosciaena crocea Richardson. The results will have positive effects to the study of three economic animals'phylogenesis, germplasm resources and genetic diversity.The Inter Simple Sequence Repeat (ISSR) technique was applied to assess the genetic variation and the genetic differentiation among three populations of Coelomactra antiquate, the Jiangsu Ganyu (GY) population, Qidong (QD) population and the Fujian Changle (CL) population. Under predetermined optimal reaction conditions, 236 sites including 234 polymorphic sites were detected ranging from 200 bp to 3000 bp with 17 primers. The number of amplified bands ranged from 8 to 20 in every individual. The proportion of polymorphic loci (PPL) of GY,QD and CL was 94.92%, 86.86% and 90.25%, respectively; value of Shannon diversity in GY,QD and CL was 0.5419, 0.4918 and 0.4895, respectively; Value of Nei's genetic variation in the three populations was 0.3700, 0.3361 and 0.3301, respectively. It's shown that all the three groups have a high level of genetic diversity; the clustering analysis showed that the nearest phylogenetic relationship occured between the GY and QD populations and then posed a sister branch to the CL population.The Inter Simple Sequence Repeat (ISSR) technique was applied to assess the genetic variation and the genetic differentiation among three populations of Pseudosciaena crocea Richardson, the Jiangsu (JS) population, Zhejiang (ZJ) population and the Fujian (FJ) population. Under predetermined optimal reaction conditions, 78 sites including 74 polymorphic sites were detected ranging from 200 bp to 3000 bp with 7 primers. The number of amplified bands ranged from 8 to 14 in every individual. The proportion of polymorphic loci (PPL) of JS,ZJ and FJ was 75.64%, 65.38% and 80.77%, respectively; value of Shannon diversity in JS,ZJ and FJ was :0.4610,0.3909 and 0.4758, respectively; Value of Nei's genetic variation in the three populations was 0.3183, 0.2697 and 0.3592, respectively. It's shown that all the three groups have a high level of genetic diversity; the clustering analysis showed that the nearest phylogenetic relationship occured between the JS and FJ populations and then posed a sister branch to the ZJ population.