The Study Of Chinese Cabbage Resilience Method Of Genetic Transformation

Chinese cabbage (Brassica campestris L.ssp.pekinensis) in Asia, especially China, Japan and South Korea is the most important vegetables. In this study, Chinese cabbage 4 homozygous parents and their hybrid offspring of the cotyledon petiole explants for the establishment of a high frequency in vitro shoot regeneration. Analysis of the control of plant shoot regeneration ability from genetic factors.Using Agrobacterium-mediated transformation in vitro techniques to betaine synthesis gene BADH and drought gene transcription factor gene for the purpose of KT383 to cabbage "Beijing 80" and the "New Beijing No.2" as the test material was Experimental studies in vitro transformation.Using Agrobacterium-mediated in situ conversion technologies. Bacillus thuringiensis (Bt) genes and the protease inhibitor gene synthesis of bivalent insect-resistant Bt-Pin II gene for the purpose of gene to cabbage high generation of homozygous inbred lines:09-482; 09-1070; 09-1073; 09-1074 initiated inflorescence dipping Transgenic Research.Bacillus thuringiensis (Bt) genes and the protease inhibitor gene synthesis of bivalent insect-resistant gene for the Bt-Pin II gene, carried out jointly deal with ultrasonic+vacuum infiltration transformation of germinating seeds of the experimental method. Harvest the seeds from the cross, transgenic plants in the seedling stage to screen, big Baotou, Beijing 80, JingXiaWang's offspring has not been the PCR positive plants, Beijing3 generations of new access to 30 PCR positive plants, preliminary results show that, Bt-Pinâ…¡gene was integrated into the Chinese cabbage genome.