Functional Analysis On The Three Related Genes Of Arabidopsis Thaliana In Responding To Phenanthrene Stress

It is reported that polycyclic aromatic hydrocarbons (PAHs) is harmful to human health, causing monstrosity, cancer and mutation. In recent years, the content of PAHs increases dramatically in the soil and other environment as the development of booming economic growth in China,especially the wide use of fossil fuels. Plants can absorb and transport PAHs from air, water and soil. At present, the molecular response to PAHs stress in plant is not clear yet. In this study, we conducted the function analysis of three candidate genes, which were selected on the basis of the differential proteomics studies to phenathrene stress in Arabidopsis thaliana. The approach of gene activation and inactivation was applied through constructing overexpression vectors and the T-DNA insertion mutants. The main results were as follows.Three over expression vectors (AT1G18080, AT1G65980, AT3G16420) were constructed, and transformed into Arabidopsis thaliana Col-0 plants with Agrobacterium tumefaciens -mediated transformation method. The semi-quantitative PCR was then used to screen and identify the overexpression plants of two genes(AT1G18080, AT1G65980). Results showed that one line of overexpression plant of AT1G18080 and AT1G65980 was more sensitive to PAHs stress on root length, which is shorter than that of wildtype. However, the plants of the loss of function T-DNA insertion mutants had no obvious differences from the wildtype. These results suggested that AT1G18080 and AT1G65980 may be involved in responding to PAH stress in Arabidopsis thaliana, however, further function alaysis should be conducted.