Resistance Evaluation Of Fujian Rice Germplasm Resources To Sheath Blight And Broad-Spectrum Resistance Of Transgenic Rices

Rice sheath blight, caused by Rhizoctonia solani, which makes huge damage to rice production around all the world, has become the most serious disease in southern rice producing regions in China. Selection and breeding of rice resistant vatieties are the most economical and effective measures to control the disease. To evaluate the resistance of Fujian rice germplasm resources, in the previous study, the inoculation methods at the seedling stage were established, and a set of R. solani isolates were screened out to evaluate the resistance. In this study, the paddy's inoculation method was used to evaluate the resistance of Fujian rice germplasm resources against sheath blight. A total of 363 rice varieties and lines were evaluated. The results indicated that the resistance of Fujian rice resources to R. solani was rather low. No high-resistant (HR) or resistant (R) varieties were found. Only 10 varieties showed moderate resistance (MR) to the fungus. The investigation results will contribute to control and prevent sheath blight, and provide theoretical basis and resistant varieties for resistance breeding.OsRBCS and OsUBC2, which were induced by the infection of sheath blight fungus, were screened out in the previous work. These two genes together with MGG₀7965.6 which belongs to the serine protease superfamily in Magnaporthe grisea were all successfully respectively transformed into rice rice Minghui-86, and transgenic plants were obtained. Resistance evaluation found that partial transgenic lines showed enhanced resistance to rice blast, sheath blight, bacterial leaf blight and bacterial leaf streak in different degree. The results indicated that broad-spectrum resistance of the transgenic lines were acquired, and these genes were closely related to rice defence response to diseases.