Dynamic Study Of AM Fungi Diversity And Glomalin In Diffrernt Leymus Chinensis+Stipa Grandis Communities

The distribution of Arbuscular mycorrhizal fungi (AMF) is extensive in the natural world, AMF are common in grasslands.The composition and diversity of AM fungi has great significance in adjusting the construct of the plant community, determining the diversity of plants and the productivity of ecosystem and maintaining the function and stability of ecosystem. This paper is helpful to the research on AM fungi diversity and glomalin in Leymus chinensis+Stipa grandis communities.It will further uncover the change and function of AM fungi in different degraded pant community.The findings are as following:1.Isolation and purification of the AM fungi led to the identification of 23 species in 5 genus,6 species belonged to Acaulospora,2 species belonged to Diversispora,1 species belonged to Entrophospora,12 species belonged to Glomus,2 species belonged to Scutellospora. Dominant species is G.constrictum, G.geosporum, common species is D.versiforme.2. The trend of AMF changing:spore species.richness and Shannon-Weiner of different degraded Leymus chinensis+ Stipa grandis plant communities are different:undegraded>restored>severely degraded,undegraded and severely degraded higher in June and Sep, restored not much change.3.Characteristics of temporal and spatial variation of different degraded and restored Leymus chinensis+Stipa grandis communities.The content of BRSP (Bradford-reactive soil protein) is between 0.79 and 2.13mg/g, EE-BRSP (easily extractable BRSP) is between 0.29 and 1.32 mg/g.The content of BRSP and EE-BRSP are both in the trending of, undegraded>restored>severely degraded, 0-10cm>10-20cm>20-30cm in the soil depth. 4.In different degraded and restored Leymus chinensis+Stipa grandis plant communities, EE-BRSP/OC is undegraded 4.2%,restored 5.6%,severely degraded 4.5%;BRSP/OC is:undegraded 14.6%,restored 19.1%,severely degraded 15.6%.5.Spores density is significantly correlated (p<0.05) between the available nitrogen and organic matter; BRSP is significant correlation (p<0.05) between the soil water,total nitrogen and pH. EE-BRSP is significantly correlated (p<0.05) between the soil water,total nitrogen, vailable nitrogen and spores density.Spores density, BRSP and EE-BRSP increased with decreasing available P in different soil depths.Soil water, pH, total P and available P affected isolation of AMF in different Leymus chinensis+Stipa grandis communities.