| The rubber tree (Hevea brasiliensis) has been an important economic crop which has a large-scale cultivation in the tropical and subtropical regions, and 99% natural rubber comes from it. Nature rubber is important not only for the national defense but also for the industry. But the outbreak of Tapping Panel Dryness in the rubber plantations leads to a severe loss, and it has been an important factor limiting the unit yield of the nature rubber. According to the predict of rubber researchers, the production loss of nature rubber lead by TPD is 5×105t in worldwide each year that equivalent to 400 million dollars. So the exploration and research of the cause mechanism of TPD has been given more and more attention in the rubber industry, and which has important theoretical and practical significance. In this paper,rubber tree clones RRIM600 which resistant to TPD(treat) and infected by TPD(control) were treated as the experimental materials,and the proteomics research methods were used to isolate and identify the proteins related to tapping panel dryness-resistant disease.The main research results are as follows.1,The paper explore the way using two-dimensional gels electrophoresis (2-DE) to gain high resolution 2-DE profile.The results showed that TCA-acetone precipitation was applicable for extraction of rubber tree(Hevea brasiliensis) latex proteins, and using 17 cm IPG strips and silver nitrogen staining was the best path. This provides the basis for further study in latex proteome analysis of rubber trees.2,The latex proteins were extracted from the treat and control respectively in the paper.And then separated by 2-DE.1158±25 and 1173±30 protein spots were detected on the 2-DE gels of the treat and control by Imagemaster 2D Platinum 5.0 software(GE) lastly,respectively. And a total of 54 differentially expressed protein spots were detected by 2-DE analysis software, and 29 of them which were induced and regulated up in the treat sample, the other 25 protein spots were induced and regulated up in the control sample.The 36 expressed proteins which were selected from above differentially expressed proteins were analyzed by MALDI-TOF-MS, and then the MS results were used for searching online to identify and annotate all these proteins. The identified proteins involved putative NBS domain resistance protein, small rubber particle protein, beta-tubulin, putative methyltransferase, enolase 1, ATP synthase beta subunit, putative retrotransposon protein, SET domain-containing protein, Os090310800, glyceraldehyde-3-phosphatedehydrogenase, purpleacidphosphatase isoform a2, direct protoplast regeneration, ADP-glucosepyrophosphorylase small subunit. According to functional category, these proteins sorted to eight categories, including resistance and stress response proteins, and secondary metabolism response proteins, cytoskeleton proteins, signal transduction related proteins, and glucose andenergy metabolism proteins, transcription and translation related protein, basic metabolic associated protein and putative proteins. These proteins are probably related closely to resistance of TPD in rubber tree. Their functions need to be further validated. |
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