| PGPR (plant growth-promoting rhizobacteria) are beneficial bacteria which can colonize in rhizosphere, directly and indirectly promote crop growth and inhibit plant diseases.In this study, rhizosphere microbes were isolated from the corn rhizosphere through selective medium. By using streak cultivation,22 superiority bacteria, which include 8 strains of G bacteria,6 strains of G+ bacteria,2 strains of atypical G+ bacteria, and 6 strains of uncertain G bacteria, were selected and purified.Among these bacteria,4 peudomonas strains,5 baillus spores strains are dominant bacteria. KM2 and KM19 belong to Pseudomonas.The antagonism and siderophore test on 22 strains of bacteria showed that KM15,KM16,KM17,KM18, KM19, KM3,KM13,KM12, KM5 and KM2 were capable of producing siderophores and KM19 and KM2 had broad antibacterial spectrum. Corn seed germination and plant growth showed that the two strains could promote corn growth. These two pseudomonas could be used as bio-fertilizer materials to develop new types of bio-fertilizer. |
