Genetic Diversity Of Wild Soybean(G.Soja) In Longjing In-Situ Conserved Region Of Jilin Province

In this experiment we studied the genetic diversity and relationship in wild soybean. A total of 90 wild soybean samples were collected from in-situ conserved region in Longjing county of Jilin Province by GPS positioning (collection interval is 50m), which covers the whole in-situ conserved region. According to the geographical locations, we divide all the wild soybean into three sub-populations A, B and C respectively. Furthermore, we collected 32 wild soybean samples in sub-population B for collection strategy study (collection interval is 18 m).In order to discover the genetic diversity among wild soybean and genetic relationship, the 90 samples (population(90))), sub-population A, sub-population B, sub-population C, and the 32 samples (from sub-population(32)) were analyzed by using 25 pairs of SSR markers with good reproducibility and polymorphism.The results showed that:1. By using the 25 pairs of SSR markers, we detect 138 allelic variants, on the average of 5.52. Simpson index distribution from 0.3072 to 0.8393, with an average of 0.6788. Shannon-weaver index distribution from 0.5856 to 1.9348, with a mean of 1.3663. These results show that wild soybean population(90)presents a highly genetic divergence in Longjing in-situ conserved region of Jilin Province.2. We can learn that genetic diversity of the three sub-populations have the same trend: C>B>A, and there are a great deal of differences between sub-population A and sub-population C. It suggested that genetic diversities of each sub-population is different.Wild soybean samples of sub-population B were collected in the interval of 50 m,and wild soybean samples of sub-population(32) were collected by 18 m and the genetic diversity index between them was large margin. Geographically, it means sampling methods had reflected the results, and the genetic diversity of each sub-population was distinct in the Longjing in-situ conserved region.3. The index of subdivision (Fst) among the sub-populations is 0.1419 and the gene flow (Nm) is 1.5112. These indicated that the sub-populations had about 14.19% of genetic variations. It means higher frequency of gene flow among the sub-populations, relatively.4.The coefficient of genetic similarity is between 0.56 and 0.99, with an average of 0.66. Clustering analysis shows that the distribution of the wild soybeans in Longjing is spotted, and the coefficient of genetic similarity among sub-populations has relativity between geographical distance.