Resource Investigation And Analysis Of The Genetic Diversity Of The Wild Piaycodon Grandiflourus

In the recent years,the Playtcodon grandiflourus exports increased year by year,and driven by accelerating peasants in the ChangBai Mountain Area of the excavation of the wild Playtcodon grandiflourus. Before human unused resource, the wild Playtcodon grandiflourus are facing serious damage or even faced the threat of extinction. Therefore,this research collected the germplasm resources in the wild Playtcodon grandiflourus research on the ChangBai Mountain Area. On the basis of analyzing the genetic diversity of the Playtcodon grandiflourus resource of the wild and the cultivated Playtcodon grandiflourus,and compared to protection and development of Playtcodon grandiflourus resources using for the establishment of the wild Playtcodon grandiflourus resources and reference on the ChangBai Mountain Area.1,We conducted an investigation of the habitats and growth environment of the wild Playtcodongrandiflourus in changhai Mountain area,and made an collection of the germplasm resources at 24 points in this area. In the survey,we found that most of the existing wild Playtcodongrandiflourus ,which survive because of its small.roots that means no value to excavate or remote location that leads to much difficulty to dig,grow in relatively poor condition or at quite remote or deserted places.2,To 24 points from the changbai wildlife habitats and growth of the wild Playtcodon grandiflourus,on the basis of that,collected some seeds are planted in experimental determination of agronomy,and measured wild plant height of eight main agronomic characters of variations,comparison with 24 cult ivationPlaytcodon grandiflourus. Results showed that grow in the testing of wild plants grow strong, the plant-height eight main agronomic characters of variation and cultivation, the similar wild and cultivated Playtcodon grandiflourus between still not obvious difference,some wild Playtcodon grandiflourus germplasm resources in the production of Playtcodon grandiflourus can directly applied.3,Comparison analysis of all eight copies the wild Playtcodon grandiflourus and cultivation Playtcodon grandiflourus that examination of the different sources of supply of which height campanulaceae eight major agronomic traits such as genetic diversity. The results showed that: the comparative analysis of eight agronomic traits in wild Playtcodon grandiflourus and cultivation Playtcodon grandiflourus genetic diversity both within campanulaceae,and the result of the analysis of variance of most traits have a significant or hightly significant differences,but there is no significant difference in the wild Playtcodon grandiflourus and cultivation Playtcodon grandiflourus except the diameter,And on the frequency distribution maps mutual cross-distribution,description of the comparative analysis of eight agronomic traits has a certain degree of similarity between he wild Playtcodon grandiflourus and cultivation Playtcodon grandiflourus.4,Detemination of the plant with the experimental materials for 8 copies in the annual of the wild Playtcodon grandiflourus and cultivated Playtcodon grandiftourus on test-bed for the College of Agricultural cultivation,and Playtcodon grandiflourus root of colorimetry were total ginsenosides. Theresults show that: the wild and cultivated Playtcodon grandiflourus has certain differences on various quality resources between total ginsenosides,the cultivated Playtcodon grandiflourus for the average total ginsenosides 2.664%, the wild Playtcodon grandiflourus average total ginsenosides in this test for 2.517%,but had no significant differences.5,As the experimental material to Playtcodon grandiflourus .The establishment of a suitable relatively stable plant SRAP reaction system results show that the campanulaceae SRAP amplification system as the best template DNA 20ng,0. 8μmol/L primer,dNTPconcentration 150μL,,MgCl2 2. 0 mmol,1 U Taq polymerase,10×Taq polymerase buffer at 2.0μL in the 20μL reaction volume. The optimum SRAP reaction procedure was: predenaturation at 94℃for 5 min followed by 5 cycles of denaturation at 94℃for 1 min,anneal at 35℃for 1 min and extension at 72℃for 1 min; then 35cycles of 94℃for 1 min,50℃for 1 min and 72℃for 1 min; and a final extension at 72℃for 5 min; kept at 4℃. A high-reproducibility was obtained with the optimized experiment condition.6,Using SRAP molecular marker technology,eight were wild and eight copies of germplasm resources of cultivated Campanulaceae polymorphism in the genetic material results of the study showed that twelve primer pairs produced 109 bands, veraged 9.1 bands per primer pair,and produced 80 polymorphic bands,veraged 6.7 polymorphicbands per primer pair. Showed higher rate of polymorphic (73. 4%). The SRAP data obtained genetic similarity range 0.56-0.88 ,The results showed that the 16 samples of Platycodon grandiflorum DC were clustered into the wild Playtcodon grandiflourus and the cultivation Playtcodon grandiflourus,two groups at genetic distance 0.69.