Primarily Studies On The Interactions Between Their Host Plants, The Green Rice Leafhopper, Nephotettix Cincticeps And Rice Dwarf Virus (RDV)

I studied the effect of rice dwarf virus (RDV) on reproductive physiology, combined with biological characteristics and molecular biology techniques to study the changes of reproductive physiology after leafhoppers infected with RDV. This reveals the potential spread of RDV mechanism. Be summarized the main content as follows:1. Effects of RDV on biological characteristics of leafhopperCompared with healthy rice plants, longevity and fecundity of leafhopper feeding rice plants infected with RDV increased. After the rice plants infected with RDV rice by altering the physiological state, in the early to attract black-tailed leafhopper feeding, and then spread to the healthy rice plants, increasing the prevalence of rice dwarf virus. RDV infection in healthy rice plants and rice plants on the feeding of egg number and egg mass was no significant difference. In addition, leafhopper had to high resistance to organophosphate insecticides, like chlorpyrifos and dichlorvos, but leafhopper had to high sensitivity to new pesticides, indicating RDV on the leafhopper resistance/resistance could promote the occurrence and development.2. Alternations in ovarian development and fecundity of leafhopper after feeding on rice plants infected with rice dwarf virusFollowing to observe the general morphology and developmental characteristics of ovaries, alternations in ovarian development and fecundity of the green leafhopper, Nephotettix cincticeps (Fabricius) after feeding on rice plants infected with rice dwarf virus (RDV) were studied. By comparison with feeding on healthy rice plants, the number of ovariole, total number of oocytes and the proportion of oocytes developed at the grade I status in pairs of ovaries of the leafhopper adults feeding on RDV-infected rice plants became to be significantly increased although the ovariole length and the proportion of oocytes developed at the grade III status became to be shorter or lower and the proportion of oocytes developed at the grade II and IV status showed to be markedly unchanged. Thus, it means that the potential fecundity of the leafhopper can be accelerated when feeding RDV-infected rice plants. Similarly, the leafhopper significantly increased its fecundity when feeding RDV-infected rice plants. In conclusion, it is suggested that RDV itself through infecting rice plants could significantly influence the ovarian development of the leafhopper and accelerate fecundity of the leafhopper.3. Detection of mating and sperming transmitting RDV with leafhopperThe study found:through mating, the female can transmit the virus to male and male can not be spreading the virus to females. Leafhopper male adult testis existed RDV virus particles under electron microscope, but can only see the electron micrograph of RDV incomplete development of virus particles. To RDV P2 coat protein antibodies, using Western-blot method to detect male adult testis of infected cases, the result is not found to contain the virus coat protein in the RDV particles, indicating that the sperm found in Cincticeps the RDV virus particles did not pass drug capacity, and can not mating adult female transmission of the virus can not spread to future generations.