| The application of mixture of pesticides is very population on chemical control of plant diseases for the time being, it is important in varieties of complex fungicide in pesticide market. Rational use of complex fungicide, first can achieve better than a single dose of fungicide control effect, the second can be achieved the objective to rid of diseases, the third can reduce the risk of pathogen resistance. However, complex fungicide is based on what, which types of fungicides were selected to complex, how to use complex fungicide in the production, and lack of the deep research. In view of these issues and the broad application of complex fungicide, screened a single dose of complex system at first in this study, the jointed effects of the different proportions of binary complex system on target, the jointed effects of different proportion on biological to pathogens, compared physiological and biochemical properties of synergistic and antagonistic complex system to pathogens, compared physiological and biochemical properties of resistant isolates and sensitive isolates to complex system and other exploration on the mechanism:1. Toxicity tested on single fungicide on Fusarium graminearum and Botrytis cinerea.The toxicity was various of different mechanisms of fungicides against Fusarium graminearum.Triazole on Fusarium graminearum that had a certain inhibitory ;Benzimidazole on Fusarium graminearum also had a certain effect; A acid phenyl ester was basically null and void; Amide, Carbamate, Pyrimidine had been largely ineffective; Antibiotics (Zhongshengmycin) had a certain inhibitory effect; Oxazoline was also inhibited. Different mechanism of fungicides against Botrytis cinerea that inhibition were also different.2. Thiram were selected to complex with tebuconazole on Fusarium graminearum,Thiram were selected to complex with SYP-Z048 on Botrytis cinerea, to confirm the synergy and antagonistic complex system.Measured in different proportions, 26 of the all proportions which had a very good synergy to Fusarium graminearum of resistant strains and sensitive strains. Thi7+Teb10 was the most synergy to two kinds of different strains; Thi9+Teb4 was the most antagonistic; the explain of synergy and antagonist tested that selected Thi7+Teb10 and Thi9+Teb4.Measured in the different proportions, 15 of the all proportions which had a synergy to Botrytis cinerea of resistant strains and sensitive isolates. That Thi5+SYP1, Thi5+SYP2 synergies outstanding performance in the all proportions, but the Thi5+SYP1 was stronger synergy to resistant strains, after the explain of synergy tested on Botrytis cinerea that selected Thi5+SYP1.3. Measured in biological characteristics of the pathogen in the jointed effect of fungicide complex system.The results showed that the synergy complex system could strongly inhibit the growth and development of the pathogens, primarily by inhibiting spore germination and mycelial growth , to reduce the number of spore formation, spore germination rate and to reduce the number of sclerotia .4. Compared the different physiological and biochemical properties of synergy and antagonistic complex system to disease.Synergy complex system that treated mycelium, which trend of the cell wall osmotic pressure was correlated with different single-dose and the control, but also resulted in reducing sugar, chitinase, soluble protein, sterol such as leakaged of inclusions. And the antagonistic complex system of the results were basically consistent with the treatment of control.5. Compared the different physiological and biochemical properties of synergy complex system to different resistance strains .The synergy complex system which treated different resistance strains ,cell wall were subject to varying degrees of damaged, that results was one of the synergy reasons. And its ultimate reason was the enzyme in vivo (extracellular enzyme) activity increased that leading to cell wall damaged and inclusions content changed.6. Discussion on the evaluation of reasonable complex system.
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