Effects Of 17-β-estradiol & Progesterone On The Expression Of Cyclooxygenase In Bovine Oviduct Epithelial Cells

Cyclooxygenase (COX) is a rate-limiting enzyme for animal tissue produced prostaglandin. It has two currently known isoenzymes COX-1 and COX-2. Many research results show that prostaglandin is a local hormone performing a wide range of functions in mammals. It plays an important role in many stages of the birth process such as ovulation, fecundation, labor and rooting, etc., including the regulation of fallopian tube functions. Studies into the adjusting function of PG have mainly focused on COXs, and there has been no report about research on the interrelations between the COX expression of female oviduct epithelial cells, and estrogen and progesterone. In order to clarify how estrogen regulates COX expression of female animal oviduct epithelial cells, this research adopts the real-time RT-PCR and Western blot methods. BOECs are cultivated extra-corporally and method to establish the regenerating cells is found. Extra-corporal method is used to discover the impact of estrogen and progesterone on the COX expression of female animal oviduct epithelial cells. BOECs cultured in vitro are of different concentrations.17β-estradiol and progesterone are treated at different times. The purpose is to find out the interrelation between the expressed amount of COX and estrogen as well as progesterone. Study results show adding 17β-estradiol of different concentrations, and after 24 hours of culturing, COX-1mRNA reaches the highest point in 100pg/ml(10-10g/ml). COX-2 mRNA reaches the highest point in 1000 pg/ml(10-9g/ml). Copying COX-1, COX-2 and mRNA in BOECs is affected by the time when 17β-estradiol is added. However, there is no obvious regularity regarding concentration of liquid. Adding progesterone of different concentrations can help copy COX-1, COX-2 and mRNA in BOECs. COX-1runs high in 1ng/ml(10-9 g/ml)while COX-2 mRNA in 10ng/ml. Statistics acquired from adding 10ng/mL progesterone at different times demonstrate less regularity than concentration. Study results show the expression of COX-1 and COX-2 are regulated by the concentration of estrogen and progesterone. And COX-1 is more seriously affected than COX-2. 17β-estradiol makes a greater change than progesterone. This is because COX-2 is more sensitive to inducing agents. As the amount of progesterone in the subject animal is regulated by estradiol, much of its power depends on estradiol.