| The brown planthopper(BPH), Nilaparvata lugens(St(?)l) is the most important pest on paddy rice in China. Cultivating and planting resistant rice varieties are the most economical and effective method to control the BPH. However, the variation of BPH's virulence makes this great tool failure in the field condition. Thus, it is important to study the variation of the BPH's virulence. In this thesis, the feeding behaviors of two populations of BPH with different virulence on different rice varieties were studied. The proteins of two populations with different virulence on different rice varieties were separated respectively by two dimensional- polyacrylamide gel electrophoresis (2D-PAGE).The main results are as follows:1 Comparison studies on the electrical penetration graph (EPG) of two populations of BPH with different virulence.The EPG results showed that the population of BPH and rice variety had most significant effects on duration of I waveform, followed by A waveform, then S waveform. And the sex of BPH did not affect the three types of waveform. The EPG of two populations of BPH with different virulence (TN1 population, reared on rice variety TN1 for more than 140 generations; Mudgo population, reared on Mudgo for more than 140 generations)on susceptible rice variety TN1 and resistant rice variety Mudgo (with resistance gene Bph1) were studied. Three treatments represent being virulent (TN1 population on TN1, Mudgo population on TN1 and Mudgo) showed shorter S and A waveform, compared with that of non-virulent treatment (TN1 population on Mudgo). And the difference of A waveform between virulent treatments and non-virulent one was significant. The I waveform of three virulent treatments was significantly shorter than that of non-virlulent treatment. We also found the accuracy to distinguish the virulence of TN1 population and Mudgo population were 82.6% and 100%, respectively,when 75 min of I waveform in 3 h record period was used as a criterion, i.e., more than 75 min represented virulent, less than 75 min represented non-virulent. It is suggested that the duration of I waveform is possible to use to develop a new method to monitor the virulence of individual BPH.2 2D-PAGE anaylsis of two populations of BPH with different virulence on different rice varieties.The 2D-PAGE anaylsis results indicated that, the profiles of proteins in four treatments were significantly distinctive.Based on comparing the different sample preparation methods and staining methods, a set of 2D-PAGE procedure suitable for BPH proteins were selected.In this paper, by using this 2D-PAGE procedure, the differences between four treatments of BPH proteins were separated respectively and compared. These four treatments of BPH included T-T (TN1 population reared on rice variety TN1 for more than 140 generations), M-M (Mudgo population reared on rice variety Mudgo for more than 140 generations), T-M (T-T reared on rice variety Mudgo for 1 generation), M-T (M-M reared on rice variety TN1 for 1 generation). There were 55 spots different in expression in all, including specific proteins and different proteins in quantity. There were 18 spots different in expression in three virulent treatments (TN1 population could be virulent to rice variety TN1, Mudgo population could be virulent to rice variety TN1 and Mudgo), and 3 spots in non-virulent treatment (TN1 population reared on rice variety Mudgo for 1 generation). And there were one spot different in expression in two treatments (T-M, M-M). In addition, there were 5 specific proteins just in T-T. These spots would be perhaps related to the variation of the BPH's virulence in rice varieties. Therefore, to further clarify the virulence mechanisms related to proteomics, it is necessary that all different proteins are selected to be analysed utilizing mass spectrometry, and the relationship between the virulence mechanisms and the function of different proteins are made clear.
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