The Study Of The Structure Of The Polysaccharides From Mirabilis Jalapa L.

Mirabilis jalapa L., which is considered of great value in traditional medicines of China, has already come into widely use in medicine and research with its various medicinal effect. As a member of Nyctaginaceae. , it is widely distributed over large areas, like in the south and north area in China. It is reported that Mirablis jalapa L. have various meidical functions, such as anti-cancer, regulate blood sugar level, antidote to heat and promote blood circulation effects. From Mirabilis, such as stigmasterol,β-sitosterol and trigonelline have been founded to be some positive functional constituents. Moreover, there is no study about polysaccharides which is isolated from the roots of Mirabilis japala.In this research: water-soluble polysaccharide extracted from 0.5mol/L NaOH from Mirabilis jalapa roots(MJL), has been isolated with hot water treatment. After freezing, trawed and removing floating protein and other impurities,Mirabilis jalapa polysaccharide was rated by different consistency of alcohol and extracted by Sepharose CL-6B gel filtration chromatography. Moreover, the structure of Mirabilis jalapa roots polysaccharide was analysed. MJL, whose molecular weight is about 42 kDa, is an acidic polysaccharide , composed of Rha,Man,Glc and Gal, percentage of each sugar is 2.47:2.50:8.47:2.1 .After partial acidic hydrolysis, periodic acid oxidation, Smith degradation ,GC-MS analysis and IR analysis ,the main structure of MJL appeared to be : The main strain of MJL is composed of (1→6)2,3,4-Gal ,(1→6) 2,3,4-Man ,and (1→4) 2,3,6-Glc and( 1→2,4)3-Rha. makes the branch part attached to (1→4,6) 2,3-Glc和(1→3,6) 2,4-Gal,terminal part of the main polysaccharide structure is( 1→)2,3,4,6-Glc.