Construction Of Expression Vector AtWRKY70 With Disease Inducible Promoter LURP1 And Its Rice Transformation

LURP1 (late upregulated in response to Hyaloperonospora parasitica) , a sustained rise of resistance gene in the late of a fungal infection in Arabidopsis. LURP1 promoter plays an important regulatory role in Arabidopsis resistance to fungal disease. Research confirmed that plant-specific transcription factor AtWRKY70 is an important member in SA and JA-mediated signal pathway. AtWRKY70 expression induced by SA, but inhibited by JA. AtWRKY70 regulated by SA is non-dependent NPR1. By constitutive overexpression of AtWRKY70 increased plant resistance to pathogens.But it not clear that put LURP1 and AtWRKY70 together whether have a much stronger resistance to the disease in rice. This study aimed to construct the related vectors then transfer them into rice and obtain the transgenic plants. Furthermore, the study laid the foundation for the further researches on LURP1 promoter and AtWRKY70 transcription factor function analysis in rice. The main results of this study are as follows:1, constructed the LURP1 inducible promoter binary vector. Using this binary vector constructed two expression vectors through Gateway technology.One is LURP1 inducible promoter and gusA fusion protein expression vector, the other is expression vector of the transcription factor AtWRKY70 driven by LURP1 inducible promoter and completed the sequence test of the two genes.2, launched a research on the Agrobacterium-mediated genetic transformation of rice.Obtained transgenic plants and identified 13 pMLURP1:: GUS and 11 pMLURP1-WRKY70 cDNA transgenic plants by PCR test.These results laid the foundation for identifying the resistance functions of the inducible promoter LURP1 and AtWRKY70 transcription factor in rice, screening bioactive small molecules and researching promoter and transcription factor interaction.