The Construction Of TiERF1-specific Expression Vector And Its Transformation Into Wheat

With the development of wheat production and the expansion of cultivated area, wheat disease is also more and more prominent. Genetic engineering to improve disease resistance of wheat raises widely attention.In this study we first cloned a phloem-specific expression promoter RSSP1 from the rice variety "RiBenQing" genome. Sequence analysis showed that the sequences of the cloned molecule are the same with the published sequence within the main function elements. It demonstrates we cloned the phloem specific promoter RSSP1. The promoter was recombined to plasmid pAHC20 with a disease resistant relative transcription factor that previously cloned from Thinopyrum intermedium so that the pA20-RSSP1:TiERF1 vector is constructed. It enables the disease resistance gene can only be expressed in phloem in the transgenics. The recombinant was transformed into wheat "Yang Mai 12" by particle bombardment transformation and regenerated wheat transgenic plantlets obtained. With two generations analysis and screen of the TO and T1 transgenic plants by PCR some 23 transformed plantlets from TO and 57 transformed plantlets from the T1 identified.The transgenic wheat provide a good material sources for screening of the disease resistant wheat while the application of a phloem-specific expression promoter can improve the food safety of transgenic wheat.