The Study Of Soil Microbial Changing In The Evolution Of Broadleaved Korean Pine Forest

To understand the change in microbial functional diversity and its impact on Pinus koraiensis update in the secondary succession of original Pinus koraiensis forest, original Pinus koraiensis forest which has the similar site conditions in the core area of Liangshui National Nature Reserve of Yichun in Heilongjiang Province and broad-leaved forest which has Pinus koraiensis update in recent years(each three), rhizosphere soil of three Secondary broad-leaved forest in surrounding forest (the rhizosphere soil in Original Pinus koraiensis forest is pine rhizosphere soil, the rhizosphere soil in secondary broad-leaved forest is Updated Pinus koraiensis seedlings rhizosphere soil),non-rhizosphere soil,were selected as study soil. Soil microbial functional diversity, soil nutrients and their relationship of forest soil 0-10cm layer of these two types were studied with Biolog and soil chemical analytical methods. The results showed that:Broad-leaved forest soil had an absolute advantage both on the content of organic matter and total nitrogen, while Pinus koraiensis soil was dominant on the inorganic nitrogen.In rhizosphere soil, the broad-leaved Korean Pinus koraiensis forest also holds certain advantages on organic matter and total nitrogen, and on inorganic nitrogen, the dominated situation also appeared.Broad-leaved forest soil was slightly higher than original Pinus koraiensis forest on the overall microbial activity. Although the overall activity is similar, its impact factors were inconsistent, through analyzing correlation among every index, such as microbial activity, organic matter, and so on. Microbial activity of Original Pinus koraiensis forest soil and ammonium nitrogen were significantly related, which showed Microbial also displayed some favor of amine in the original Pinus koraiensis forest which ammonium was dominant in, whereas that did not exist in the broadleaf forest. In rhizosphere soil, the microbial activity was quite different, AWCD values of Pinus koraiensis seedlings'rhizosphere soil in Broad-leaved forest were almost 25% higher than the original Pinus koraiensis forest.Based on overall richness and diversity index, the original Pinus koraiensis forests' rhizosphere was dominant both on diversity and abundance,but its soil was at the lowest level, which indicated that in primary forests, micro-organisms were more concentrated in the vicinity of roots, the root exudates had an greater impact on the microbial.By studying the utilization of overall carbon with the PCA, it was found that four types of soil were different on using of carbon source.This difference is not very obvious in soil, but not in rhizosphere soil.While with the pine rhizosphere soil, microbial species would be different, because of the cover from different advantageous in vegetation (original Pinus koraiensis broad-leaved forest, pure white birch).Through the variance analysis of single carbon sources, it was found that in the original forest, whether soil or rhizosphere soil, all had Great advantages in the utilization of Amino acid carbon, which confirmed each other with the pine's favor of ammonium. In the rhizosphere soil of pine seedlings of Broad-leaved forest, the microbes are not dominant on using of amino acids, which showed advantageous micro-organisms would have been changed in the rhizosphere microhabitats.In general, in the secondary succession of temperate forests,the soil microbial functional diversity did have been changed, and developed towards the disadvantageous aspects of Pinus koraiensis regeneration.