Construction And Analysis Of An Inducible Insect Resistant CDNA Library And Its Expressed Sequence Tags From Jasmonic Acid-treated Larix Gmelinii Needles

The Larix gmelinii leaves were induced by jasmonic acid was used as experimental material, and total RNA was extracted from the leaves, cDNA was synthesized and ds cDNA fragment was ligated into the pDNR-LIB vector. The recombinant plasmid was transformed into Escherichia coli DH5a by electroporation. The library qualification evaluation showed, The library had 5×106 independent clones,0.5-2 kb fragments with an average of about 1 kb in length, recombination rate of 95%. cDNA library of the L. gmelinii needles were induced by jasmonic acid was constructed successfully.Sequenced 980 independent clones, out of which 812 clones produced clean expressed sequence tags (ESTs) for gene expression analysis. Computational analysis of 750 non-repetitive sequences with the known GenBank database identified a number of induced plant resistance-related genes such as heat shock proteins, plant phenylalanine ammonia-lyase (PAL), and cysteine protease inhibitors.The dbEST and the associated DNA genetic resources in this study will be a useful resource for the further study in understanding the molecular mechanisms of JA-induced plant insect resistance.