The Proteomic Analysis And Comparison Of Pathogen Defense Associated Physiological Active Matter Of NILs Taichung29*6/Yr5 Resistant To Wheat Stripe Rust

Wheat (Triticum aestivum) is a main crop planted about 4 million hectares, and with yield about 22% of national grain output in China. However, the wheat stripe rust (Puccinia striiformis f.sp.tritici) , a major disease of wheat, has been a serious threat to China's wheat production and food security. In recent years, many researchers have done a lot of research in wheat stripe rust resistance gene, such as genetic analysis of resistance to wheat stripe rust, mapping of major resistance genes and QTL. Since wheat is allopolyploid, wheat genomics is highly complex with 80% repeat sequences in it. Wheat stripe rust is a popular of parasitic fungi which sexual stage has not yet been found. These characteristics bring about great difficulty in researching the pathogenesis and resistance mechanisms. It is important theoretically and practically to study the resistance to wheat sripe rust and the interaction between wheat and P. striiformis f.sp.tritici.In the research, the quantity of chlorophyll a and b in leaves of wheat NILs Taichung29 and Taichung29*6/Yr5 inoculated with stripe rust race CY32 was examined by the methods of chlorophyll color comparative,absorption spectra scanning and optical absorption. The result shows the ratio of chlorophyll a / b in wheat leaf increased after infection by stripe rust CY32.By the methods GC-MS and relative quantification technology, the content of secondary metabolites in leaves of Taichung29 and Taichung29*6/Yr5 after infection by stripe rust CY32 was measured. Comparison of results showed that, after infected by stripe rust CY32, contents of 37 and 27 species secondary metabolites respectively in leaves of Taichung29 and Taichung29*6/Yr5 increased, and contents of 55 and 52 species econdary metabolites respectively in leaves of Taichung29 and Taichung29*6/Yr5 decreased.Using the methods of GC-MS and Selected Ion Monitoring (SIM), I studied plant hormone NAA, IAA and ABA. By comparing the content, I found the content of NAA and ABA was up and the content of IAA was down.The technology of subcellular and multi-stage separation technology were used to separated and purified the intact chloroplast. Then the membrane protein complex of wheat chloroplast was purified and preliminary analysed by BN-PAGE. the ATP synthetase and PS-II reaction center D2 were identified by Peptide Mass Fingerprinting(PMF).