The Analysis Of Breeding Value And Development Of Inbred Strain For The Wild Mice In Henan Province

Laboratory animal has been used for 100 years and play an irreplaceable role in the research of biology. Inbred mice were being used widely in many fields such as genetics, physiology, pharmacology, medicine, veterinary and so on because of its unique advantages. But with the deeply increasing development on genetics and related fields, the common inbred strains which were mostly bred from European strain represent some defects such as the simplex DNA structure, limited biological diversity, it was inconvenient for the application of molecular biology methods in gene detection and other aspects. Thus researchers focused more and more on the exploitation of laboratory animal derived from different wild mouse species or subspecies, and breeding of inbred strains from wild mice, and these would become a new, actively research field gradually.This experiment aimed at capture and domestication of wild mice in Henan Province, and analysis of the value of their breeding animals of the preliminary development. There were 25 wild mice captured in the experiment which were compared with the common laboratory inbred mice in body size. In order to estimate the breeding value, microsatellite markers were used to analyze the differences in the genetic composition. The domestication of wild mice and inbreeding process were making basis for the establishment of inbred strain.1. There were 25 wild mice captured (14 males and 11 females). All the captured wild mice belong to the Mus musculus species through the morphologic identification, it is the desired result for the experimental design2. In order to domesticate the wild mice, some measures were performed: breeding separately, using sawdust mixed with wastepaper as bedding pieces, shading with wood box, feeding appropriately with corn and chicken feedstuffs , lighting regularly, keeping steady temperature , and avoiding to disturbing them. Two months later, except one mouse death due to stress, the remaining wild mice survived, and follow-up experiments can be continued.3. Compared with body size, the wild mice were significant smaller than BALB/c mice in Body weight, body length, tail length, hind foot length and ear length. 4. 56 microsatellite markers were used for detecting the genetic polymorphisms between wild mice and common laboratory strains (PWK, BALB/c, C57BL/6, KM). The results indicated that there were high polymorphisms frequency between wild mice and common laboratory strains, and it was useful for enrich the mice gene pool.5. The breeding of inbred mice: putting the male into the female cages or feeding together into a new cage in another way. Setting seven mating routes, in which B, C and F group litter respectively, and then set the three groups as the main route, each route were separate with two lines as extensions. Breed under the principle of full sibling mating, and the reproduction data were recorded toughly. At present the reproduction of each group is normal, group B and C has spread to the fourth generation, group F has spread to the third generation.