Effect Of Bacillus Subtilis On Growth Performance And Intensive Aquaculture Environment Of Cyprinus Carp

In the experiment 375 Cyprinus carp were allotted into five groups randomly for 90 days . The first group was the control group fed with basal diet, the others were the treated groups fed with basal diet addited 0.1‰, 0.2‰, 0.3‰, 0.4‰Bacillus subtilis(2×109cfu/g) respectively. The weight gain, activities of protease and amylase of the intestine, chyme and hepatopancreas, Hepatopancreas Somatic Indices were determined. The results showed Bacillus subtilis of 0.2‰, 0.3‰were the optimum growth for Cyprinus carp. The growth ratios of tested groups of 0.2‰, 0.3‰Bacillus subtilis were significant(P<0.05), which were 16.90%, 18.78% higher than that of the control.Compared with the control, The activities of protease and amylase of chyme of treated groups of 0.2‰, 0.3‰Bacillus subtilis were significant(P<0.05), which were 92.56%, 96.33% and 45.96%, 50.18% higher than those of the control, respectively. The amylase activities of chyme of group of 0.4‰Bacillus subtilis was 33.33% higher than that of the control(P<0.05). The protease activities in the intestine fed with dietary 0.2‰, 0.3‰, 0.4‰Bacillus subtilis were 113.34%, 156.45%, 161.05% higher than that of the control(P<0.05). The amylase activities of the intestine fed with dietary Bacillus subtilis were not significant (P>0.05). The protease activities of the hepatopancreas fed with dietary Bacillus subtilis were no-significance (P>0.05), but the amylase activities were significant (P<0.05), which were less 42.45%, 40.78%, 43.48%, 41.73% than that of the contrast. Compared with the control, the phytase activity in the intestine of Cyprinus carp fed with dietary Bacillus subtilis were 6.95%,7.77%,8.74%,8.41% higher, but no significant(P>0.05).According to the test that Cyprinus carp was fed with basal diet addited 0.3‰Bacillus subtilis(2×109cfu/g), effect on fish pond water was studied. The result showed Bacillus subtilis could reduce the concentrations of NH3/NH4+, NO2--N, COD significantly. Indications of the fish pond water on the experimental groups are better than those on the control.