Effects Of Energy Restrictions On Broiler Production Performance And Gene Expression In Liver

In order to study the 30% energy restriction on broiler production performance and gene expression in the liver, In this study, mainly carried out from the following two parts.Test one50 18-days-old Arbor Acres female broilers were used to investigate the 30% energy restriction on Growth performance, slaughter performance, cost-effective, the immune organ index and serum biochemical indicators. The chickens were randomly allotted 2 treatments (25 in each group),each bird raised in one cage. Following was the results:(1) Energy restriction resulted in 31,40 and 48-day-old broiler body weight was significantly lower than the free feeding group (p<0.01),40 to 48-day-old broilers relative weight and feed conversion rate was significantly higher than the free feeding group (P<0.01), in which the rate of relative weight gain was significantly increased 35.5%(p<0.01);(2) Energy restriction increased the rate of leg and breast muscle, leg muscle was increased by 7.9%, a significant difference (P<0.05); breast muscle was increased by 0.8%, no significant difference (p>0.05);(3) Energy restriction can reduce the fat deposition in broilers, abdominal fat weight, abdominal fat and subcutaneous fat thickness was significantly lower than the free feeding group (P<0.01);(4) Energy restricted group's spleen and bursal index was significantly lower than the free feeding group (P<0.01);(5) Energy restricted group's triglyceride and glucose level were significantly lower than the free group (P<0.05), cholesterol and high density lipoprotein cholesterol was significantly lower than the free feeding group (P<0.01).This study shows that reducing feed energy level and feed intake,a 30% energy restriction can be markedly improved broiler weight gain and relative feed conversion efficiency, increase economic efficiency and improving carcass fat deposition in broilers, improve immune function.Test twoIn order to study the 30% energy restriction on broiler liver gene expression, this experiment chose of 48-day-old healthy female AA broiler liver tissue(energy restriction and freedom group),each group 20 samples, a single individual for the repeat units. we used GenefishingTM DEG Premix Kit to select liver's differentially expressed genes, used semi-quantitative and quantitative fluorescent RT-PCR for the identification of differentially expressed genes. Following were the test results:(1) Through the GenefishingTM RT-PCR,we detected 43 differentially expressed genes; 12 differentially expressed EST were successfuliy recovery, cloning and sequencing.(2) Through semi-quantitative and quantitative fluorescent RT-PCR showed that:a total of eight differentially expressed EST, and the expression of the energy restriction group showed a downward trend.(3) Through bioinformatics analysis showed that:4 differentially expressed EST can be found highly homologous known genes,2 genes that are homologous, the other two functional genes is unknown.In this study, We screened and identified differences in energy restricted broiler liver displayed EST, This provided a reference for human researching energy restriction effects on broiler from the molecular level.