| Liquorice is an important traditional Chinese medicinal,the mainly active ingredientsare triterpenoids and licorice flavonoid,Licorice flavonoid has stronger bio-activities. Theconclusions of pharmacological investigation are that they have anti-cancer,anti-ulcers,anti-liver damage and so on,it becomes particularly important to improve the concent oflicorice flavonoid. Secondary metabolites in plant are closely related to the arid environmentof salinization where Licorice usually grows,and that is the result of the choice of long-termsurvival. According to that,the content of secondary metabolites can be increased by givinga certain degree of environmental stress.It is necessary to study the effect of abiotic stress on the growth,growth curve,physiological indicators and the liquorice flavonoid of Glycyrrhiza inflata callus. With thestress level increased,the growth of the Licorice callus drops,and MDA concentration rises.The growth of cotyledon callus aslo arises only under 0.5%NaCl stress. At first,PODactivity shows up,then downward. Under 0.5% NaCl stress,the flavonoid content ofcotyledon callus is 2.31 fold and 1.1 fold to compare with the control and the herbsrespectively. However,to compare with the control by the 0.8%NaCl and the 1.0%NaCl,the flavonoid content of cotyledon callus is 2.0 and 1.2 fold respectively. Licorice flavonoidcontent of hypocotyl callus is 2.0 fold to compare with the control by the 0.5% NaCl,and isalso improved under the ABA treatment. Flavonoid content of cotyledon callus was 1.4 timesto compare with the control by 25% PEG-6000,but it was lower than that of herbs.Chalcone Synthase (CHS) is a key enzyme in the biosynthesis of flavonoids. Usingsemi-quantitative RT-PCR method,the expression of chs gene of Glycyrrhiza inflata calluswere detected under different treatment,the results show that the flavonoid content of callusis positively correlated with the expression of chs gene.
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