The Studies On Main Factors Of Effection On Enbryoids Induction In Isolated Microspore Culture Of Chinese Cabbage And Cabbage

Fifteen different varieties were used to isolated microspore culture in Chinese cabbage and Cabbage. These factors which influence microspore embryogenesis were studied in the research. Such as genotypes, bud length, culture medium and additives, cultural conditions. The aim of researching experiment is to ensure the factors that influence microspore embryogenesis, providing the underlying basics of efficient technique system of isolated microspore culture in Chinese cabbage and Cabbage. The results were as follows:1. The genotypes was one of the most important factors of embryoids induction. Induction rate of microspore culture was significant difference in varieties of genotypes. There were 9 genotypes induced embryoids in 15 experiment materials. Among them, "Q003"and"B007"produced higher embryo yield, with 63 embryos per ten buds and 57 embryos per ten buds respectively.In addition there were 6 materials could not gain any embryos. There were far higher embryo yield in Chinese cabbage than that in Cabbage.2. The donor Plant with good nutritional status were suitable to induce microspore embryogenesis in full florescence. When the bud was between 2.5-3.4 mm long and the ratio of petal to anther was between 3/4-7/6, it is suitable for Chinese cabbage microspore culture. When the bud length was between 3.0-3.9 mm long and the ratio of petal to anther length was between 1/1-5/4, it is suitable to microspore culture for Cabbage.because the pollen was in the late uninucleate stage.3. NLN-13 medium which reduce the content of major salts by half are benefit to embryogenesis. Active carbon and agarose in medium NLN-13 with appropriate amount can promote embryoid induction and development. Embryoid growed more quickly and strongly after supplementing Agarose and active carbon in medium NLN-13. It is impossible to induce embryoid in some genotypes which were hard to induce embryoid by supplement of Agarose and active carbon. 4. There was usually one suitable content proportion of 6-BA and NAA for varieties of genotypes. "B007"can get the most embryoid induction with 0.05 mg·L-16-BA.0.2 mg·L-16-BA was needed for "Q003" to get high induction of embryoid while 0.2 mg·L-16-BA and 0.1 mg·L-1NAA for"B008".5.4℃low-temperature treatment was good for increasing the frequency of microspore embryogenesis. The optimal treatment time is 24-48h of that choice.4℃low-temperature treatment is not necessary to embryogenesis.6.33℃high-temperature treatment for some time at the very beginning of culture is necessary to embryogenesis. It can be beneficial to increase embryogenesis after 24-48h of 33℃high-temperature treatment.7. MS medium was more suitable than B5 medium for shoot of embryogenesis.