Screening And Studying On The Genes Related To Female Flower Development Of Betula Platyphylla Suk. At Different Flower Phase

Developmental stages of female flowers in Betula Platyphylla Suk.was determined by anatomical observation and biological slice making technology. As femle inflorescences were test materials, positives and reverse subtraction libraries of Betula platyphylla Suk. in flower phase were constracted.From the two libraries, unique genes were selected and were fundamentally analyzed by bioinformatics methods.Four unique genes from the two libraries were selected and detected expression in female inflorescence, male inflorescence and leaf in different development stage by real-time quantitative PCR, and the gene function was analysised preliminarily. The main results are as follows.1.Developmental stages of female flowers in Betula Platyphylla Suk.was determined. According to embryological development characteristics, the periods from 30 April to 11 May were determined as pollination, ovule nucellus,integument differentiation and Megasporogenesis stage, and the periods from 12 May to 25 May were determined as megagametogenesis and fertilization stage, and the BHHQ subtraction liabrary and the BHHZ subtraction liabrary was constructed as the cDNA of female inflorescences in the two stages used as tester and driver.2.From BHHQ subtraction liabrary,480 unique gene were selected, the redundancy is 30.64%, in reverse BHHZ subtraction library,503 unique gene were selected, the redundancy is 29.55%. Blastx and blastn analysis respectively showed that 357(74.38%) and 431 (85.69%)unique gene were homologous(Blastn E-Value