The Study On Synergistic Relationship Among Endo-β-manannase And Other Cell Wall Degrading Enzyme During Tomato Truit

Tomato(Lycopersicon esculentum Mill)is a worldwise-grown vegetable. That tomato fruits ripen in advance or postponed will produce social and economic efficiency. Softening is an obvious character and also a direct factor which is related to ediblity.The change of cell wall and degradation of cell wall materials were considered as the main factor involved in change of fruit texture, cell wall degrading enzyme degraded the cell wall material. Transferred LeMan4 in tomato through antisense RNA and RNAi showed that endo-β-mannanase was not the key factor in fruit softening.The experiment has studyed the coordination between the endo-β-mannanase and other cell wall degrading enzyme . How the plant hormone regualted the cell wal degrading enzyme .So we can facilitate the overall understanding of tomato fruit softening,the experiments results showed below.1.The experiments were measured endo-β-mannanase and other cell wall degrading enzyme of skin and pericarp of ABA-deficent, GA-deficient, microtom, ripening -inhibitor and non-ripening . In the skin of tomato , a negative correlation existed between endo-β-mannanase activity and fruit firmness with a correlation coefficient of -0.50, significant at 0.01 level;a positive correlation existed between endo-β-mannanase and PG activity with a correlation coefficient of 0.45,significantat 0.05 level:a positive correlation existedbetweenendo-β-mannanase activity and soluble solid content with a correlation coefficient of 0.53,significant at 0.01 level. In the pericarp of tomato ,the relation among endo-β-mannanase activity, cell wall degrading engzyme,soluble solid content and fruit firmness, endo-β-mannanase activity were different in differernt varity, the higher the endo-β-mannanase activity,the smaller the fruit firmness . A negative correlation existed between endo-β-mannanase activity and fruit firmness with a correlation coefficient of -0.55, significant at 0.01 level;a positive correlation existed between endo-β-mannanase activity and PG activity with a correlation coefficient of 0.48,significant at 0.01 level:a positive correlation existed between endo-β-mannanase activity and PME activity with a correlation coefficient of 0.46,significant at 0.05level: a positive correlation existed between endo-β-mannanase activity and cellulaus activity with a correlation coefficient of 0.55,significant at 0.01level: a positive correlation existed between endo-β-mannanase activity andβ-mannosidase activity with a correlation coefficient of 0.38,significant at 0.05 level: a positive correlation existed between endo-β-mannanase activity and soluble solid content with a correlation coefficient of 0.59,significant at 0.01 level. There were correlation existed among endo-β-mannanase ,PME,PG and cellulaus and effeted the fruit softening. 2.We deteminated the GA-deficient,ABA-deficient ,ripenging-inhibitor and non-ripening enzymeof cell wall degrading.GA significantly increased the activity of galactosidase, PG, mannanase, mannosidase, PME and decreasedβ-D-xylosidase activity the in the GA-deficient tomato skin and part increased and part decreased the a-L-arabinofuranosidase activity. GA increased endo-β-mannanase activity of ripening–inhibitor and non-ripening tomato skin.So GA positive regulated the galactosidase, PG, mannanase, mannosidase, PME. GA negative regulatatedβ-D-xylosidase, and negative and positive regulatied a-L-arabinofuranosidase and we speculated that the rin,nor,leman4 in the GA signal transduction . ABA coordinated with GA regulated the galactosidase activity .2. Through determinated the cell wall degrading enzyme of GA-deficient,ABA-deficient of tomato pericarp, GA significantly increased the activity of galactosidase,β-D-xylosidase ,PME and decreased the mannosidase activity and part increased and part decreased PG, endo-β-mannanase and a-L-arabinofuranosidase activity.GA decreased the acitivity ofβ-D-xylosidase of ripeing-inhibitor and non-ripening tomato pericarp. ABA coordinated with GA increased galactosidase activity .So GA positive regulated galactosidase,β-D-xylosidase ,PME and negative regulated the mannosidase and part increased and part negative regulated PG, endo-β-mannanase and a-L-arabinofuranosidase activity.GA,rin andβ-D-xylosidase in same signal transduction.GA,nor andβ-D-xylosidase in same signal transduction. ABA coordinated with GA positive regulated galactosidase .2.In tomato skin, ABA,rin,endo-β-mannanase,a-L-arabinofuranosidase,PME,PG insame signal transduction. ABA,nor ,endo-β-mannanase ,a-L-arabinofuranosidase,PME,PG insame signal transduction.GA,rin,β-D-xylosidase,PME,PG and a-L-arabinofuranosidase in same signal transduction. GA,nor, galactosidase ,β-D-xylosidase in same signal transduction.5. In tomato pericarp , ABA,rin,PG, endo-β-mannanase in same signal transduction.ABA,nor ,β-D-xylosidase,mannosidase,PG,PMEand endo-β-mannanase in same signal transduction.GA,nor, PG, endo-β-mannanase, galactosidase in same signal transduction .GA,nor,PG, endo-β-mannanase in same signal transduction.