Analysis Of Tolerance And Screening Of Rice With OsMAPK4 And OsCDPK7 Gene On Salt Stress And Alkali Stress

Rice is the world's important food crops,the 3/4 people of the world eat rice.But salinity, drought and low temperature and other abiotic stresses is the most harmful rice production and quality every year. According to statistics, the arid area and Semi-Arid Area accounts for 33 percent of the world.The damage is equivalent to the total of other natural disasters. Saline accounted for 7.6 percent of the land area. In addition, there are many areas often affected by low temperature stress.In China a total area of saline-alkali land is about 6.7 million hectares, includingmillion hectares potentials only saline-alkali land in Heilongjiang Province reached more than 9,000 hectares, has seriously hampered agricultural production in Heilongjiang Province. Relative to the changing external environment, enhance their salt and alkali resistance of plants to be more effective mitigation of the impact of salinity on the protection of the natural environment and the maintenance of world food production has an important role. Therefore, how to improve the ecological environment of plants to survive, how to improve the crop salinity, to achieve rapid agriculture, sustainable development has become an urgent need to resolve key issues.Traditional breeders have carried out long-term exploration and study to improve plant resistance,but traditional breeding methods has the problem of huge workload, long growth periods and cost more,low efficiency, the genetic resources available, through such means of genetic engineering molecular breeding avoid above shortcomings.In recent years, with studying the stress on plant molecular reactions and adversity signal transmission mechanism,researchers have found that gradually improved or increase ability to control gene regulation, is perhaps the methods and ways to more effectively improve crops resistance, the regulation of gene cloning and function is the prerequisites of plant gene engineering research, screening and obtain the transgenic plants with resistibility,and provides an important basis for the worsening ecological environment.In our study, utilized rd29A and E12 respectively promoter regulation from rice transformed with OsCDPK7 gene and OsMAPK4 gene of T2 and T3 transgenic plant as the tested varieties.Studying the preliminary screening of T2 generation of plants on salt or alkali stress for plant growth and plant death..homozygote.。Harvesting T3 transgenic plant,to the screening of homozygote and salt and alkali resistance testing, at the same time, research gene expression and the main characters of the salt and the alkali.Through this research we can be obtained transgenic rice with the ability to resist the alkali and saline, used in in Heilongjiang province. The results showed that provided a new idea to improve for plant genetic engineering modified saline and alkali, has certain theoretical and practical value. Providing an important resource for the final cultivation of normal growth of transgenic rice.The major findings are as follows:1.Genetic Stability Analysis of Transgenic Rice1.1 Genetic stability analyses of target genes in T2 generation and T3 generationThe genome DNA of T2 generation and T3 generation were determined by PCR analysis.The target genes were amplified in transgenic rice with PCR; which validated that the transgenic plants T2 generation and T3 generation co-integrated with OsMAPK4 and OCCDPK7 genes.1.2.Southern blotSouthern blot, this part of its research is in progress.2. Genetic Character Analysis to Transgenic Rice of T2 generationStudying the preliminary screening of T2 generation of plants on salt or alkali stress for plant height and plant mortality homozygote.The results showed that the variation of height of transgenic rice more than the control and the mortality rate is below the control rice Which the variation of plant height of transgenic rice which is the regulation of E12 promoters OsMAPK4,OsCDPK7 gene more than the transgenic rice which is the regulation of rd29A promoters OsMAPK4, OsCDPK7 gene.the plant mortality of transgenic Rice which is the regulation of E12 promoters OsMAPK4, OsCDPK7 gene was lower the transgenic Rice which is the regulation of rd29A promoters OsMAPK4, OsCDPK7 gene.3. Genetic Character Analysis to Transgenic Rice of T3 generationStudyed the screening of homozygote of transgenic rice of T3 generation and the screening of transgenic rice on salt or alkali resistance testing for the plant height and the index of salt or alkaline tolerance. The results showed that the variation of height of transgenic rice more than the control and the index of salt or alkaline tolerance.was below the control rice Which the variation of plant height of transgenic rice which is the regulation of E12 promoters OsMAPK4, OsCDPK7 gene more than the transgenic rice which is the regulation of rd29A promoters OsMAPK4, OsCDPK7 gene, the index of salt or alkaline tolerance.of transgenic rice which is the regulation of E12 promoters OsMAPK4, OsCDPK7 gene was lower the transgenic rice which is the regulation of rd29A promoters OsMAPK4, OsCDPK7 gene.