Preparation Of 1% Milbemycins Microemulsion

In recent years, the limit to the organic solvent for the agricultural chemicals preparation in the developed country has been more stricted. thus urged the agricultural chemicals dosage-form to renew unceasingly and develop, the water-character preparation was one of agricultural chemicals dosage-form development leading directions. Therefore, the micro emulsion and the aqueous emulsion that all or partial part the organic solvent replace by water arises at the historic moment, and it become the agricultural chemicals new dosage-form to obtain the development application.Milbemiycins is one kind of highly effective pesticide, is 16-membered ring macrolides mixtures. It is efficient on acarine and ophid. and to the vegetables, the cotton, the wheat, the paddy rice, the apple on and many kinds of harmful insects also has the good control efficiency. The insect disinfestation spectrum is broad, and there is no reporting of corresponding pesticide product in our country agricultural production. This research has developed 1% milbemycins micro emulsion, and has carried on system's formula experiment to it. Has been clear about it to the kidney bean red spider, the cabbage aphid insect disinfestation effect, and has carried on the demonstration and the promoted application.In this study, the physics and chemistry characteristic of Milbemycins have been researched systematically with chemistry and the biological method. And the surface active agent function to the Milbemycins has also been researched. Identify the product quality of 1% Milbemycins micro emulsion. The 1% Milbemycins micro emulsion has been developed successfully.The pharmacodynamic test on bean aphid and red mite demonstrate that 1% Milbemycins microemulsion were effective with both of the two field-pests.The results as following:1. In this paper, the composition and preparation of 1% Milbemycins microemulsion were introduced, which have not been reported in our country. After a serious of selection experiments of different solvent, surfactants, co-solvents, antifreeze , antiblowing, light stabilizer and water, the results showed that the optimum formulation are as follows Milbemycins 2.5%, acetidin 8%, surfactants 16% (EL-90 12.8%, pesticide emulsifier 3.2%), isopropyl alcohol 2%, ethylene glycol 5%, light stabilize1% and water. The physico-chemical property was detected. The results showed that all physico-chemical properties of Milbemycins microemulsion were well tally with the national standards.2. RP-HPLC is conducted in this study. Methanol and water are selected as mobile phase, the containing of the micellar emulsion of 1% Milbemycins microemulsion is determined. The result as following: standard deviation is 0.0030, coefficient of variability is 0.2920%, recovery rate is 99.8%, correlation coefficient is 0.9989. Its standard deviation, coefficient of variability and average recovery rate are colinearity coefficient correlation.3. The micro emulsion carried on the storing stability test according to the FAO standard to 1% Milbemycins, the normal temperature has stored 3 months, 6 months, 12 month-long rates of dissociation respectively is 1.07%, 1.43%, 1.87%; Low temperature stability (-5±0.1℃,14day), after mild vibration, does not have separates the pellet and greasy obviously, the average rate of dissociation is 2.37%; The heat stores the stability (-54±2℃,14day), the relative rate of dissociation is 1.93% equally. Mil Bei Meisu the content≥1.03%, 1% mil Bei Meisu micro emulsion each milliliter quality is the 1.0623g/ml, pH value is 5.0~6.0, transparent temperature province for - 9.6~73.4℃,1min within lasting bubbling quantity≤25 ml.4. This research uses the HPLC(high performance liquid chromatogram) to carry on the linearity analysis, the returns-ratio and the analysis method accuracy determination. After the experiment indicate: the recovery rate is 99.80%; the analysis method standard deviation is 0.0030; the coefficient of variation is 0.2920%.5. After the formula determination of 1% Milbemycins micellar emulsion, has carried on to the new development pesticide indoor toxicity and the bonsai against effect determination. Through the indoor toxicity test, obtains 1% Milbemycins micro emulsion to the kidney bean red spider and cabbage aphid's LC50, respectively is 0.0823mg?L-1 and 0.2022mg?L-1.