Combined Immunology Research Ofavain Infectious Bronchitis Virus M41 Strain And CONN Strain

Avain Infectious Bronchitis(IB) is an acute and highly infectious respiratory disease,caused by the avian infectious bronchitis virus(IBV). Since broke out in USA in the 1930s,IB have become an important disease of poultry around the world. IBV is a single-stranded RNA virus,the gene of the virus always mutated owing to it's point-mutation and gene recombination,So the serotype of IBV has more than 30 categories. Different serotypes have lower or no protection. Currently,IB has often been found in China. Even if in the farm where had vaccinate IB vaccine,the incidence is still widely. Which result in serious economic losses to the poultry industry.IBV has showed hemagglutination through treatment of trypsin, phospholipase C, culture fluidm of Clostridium perfringens and neuraminidase enzyme. However, Preparation of IBV HI antigen is very complex, and It hasn't stable and commercial IBV antigen that is made use serological detection and vaccine effective monitoring of IB at home.Results of IBV antibody are very different,as antigen titer is low, different enzyme treatment with antigen and different different preparation methods. For the better preventing Infectious Bronchitis, Which has launched unitive immunology research of infectious bronchitis virus M41 and Conn with hemagglutination inhibition test and neutralization test.We has carried on antibody discrimination with the system HI experiment. The result indicated that, through cross HI test of M41,Conn standard antigen and M41standard positive serum, Antiserum titre value were 8㏒ 2,5㏒ 2 at 21 days and had 3㏒ 2 difference, were 8㏒ 2,5㏒ 2 at 28 days and had 3㏒ 2 difference, were 8㏒ 2,5㏒ 2 at 35 days and had 3㏒ 2 difference;through cross HI test of M41,Conn standard antigen and Conn standard positive serum,Antiserum titre value were 4㏒2 ,8㏒ 2 at 21 days and had 4㏒ 2 difference, were 4㏒ 2,8㏒ 2 at 28 days and had 4㏒ 2 difference, were 4㏒ 2,9㏒ 2 at 35 days and had 5㏒ 2 difference. The proof that M41 and Conn antibody can separate out through cross HI experiment.To further confirm discrimination situation of M41 and Conn antibody, Determination of TOC-ID50 of M41and Conn virus titer with trachealis, and that carries on antiserum cross neutralization experiment. The result indicated that,Cross neutralization experiment of antiserum and M41 and Conn virus, can separate out M41 and Conn antibody obviously.Further confirmed has used accuracy with result of cross HI experiments.It has conducted Unitive Immunology Research of Avain infectious bronchitis virus M41 strain and Conn strain with HI test and neutralization experiments.The result indicated that, The antigen correlation coefficient of HI experiments (6.25%) by far was smaller than antigen correlation coefficient of neutralization experiments (3.12%), The result that neutralization experiment was more advantageous to antiserum discrimination; The antiserum titre of HI experiment and the neutralization titre of neutralization experiment is present correlation, correlation coefficient of M41 r=0.873174, correlation coefficient of Conn r= 0.843948; It must make the foundation immunity with live vaccine when makes Emulsifies antigen of IB, and that can produce high antibody; M41+Conn group,s antibody and M41 group's antibody against IB M41 strain is lower concentrate intake(p>0.05), M41+Conn group,s antibody and blank control group against IB M41 strain is higher Forage(p<0.01); M41+Conn group,s antibody and Conn group,s antibody against IB Conn strain is lower concentrate intake(p>0.05), M41+Conn group,s antibody and blank control group against IB Conn strain is higher Forage(p<0.01).