| Cryptosporidium spp. are more common agents of gastrointestinal infection in humans, domestic animals, and other vertebrates. Eight species and two genotypes have been identified in cattle, including Cryptosporidium parvum, C. bovis, C. andersoni, C. ryanae, C. felis, C. hominis, C.suis, C. suis-like genotype, and the Cryptosporidium pig genotype II. And C. parvum is the zoonotic species most commonly identified in humans.In order to investigate the infection of intestinal parasites in buffalo, a total of 357 adult free-range buffalo fresh stool from Zhangjia and Lijia stockade Village of Xinyang were conducted using Lugol's iodine staining, centrifugal precipitation method, saturated sucrose solution and modfid acid-fast stain. The results, 302 samples were positive, the total parasitic infection rate was 84.59%; Coccidium infection rate was the highest, 76.75%; Cryptosporidium, Cyclospora cayetanensis, Trematode, Strongylus, Bunostomum trigonoce phalumin, Whipworm and Tapeworm infection rate was 3.36%, 2.52%, 7.07%, 25.77%, 9.80%, 4.76%, 0.56% , respectively. The survey provided references and a basis for intestinal parasitic diseases treatment and prevention of buffalo.To establish a calf model of Cryptosporidium parvum, neonatal calves were challenged orally with oocysts C.parvum which collected in this survey. Results showed that the patent period was in 3th days post infection,and the counted number of oocysts increased obviously, its excreted peak numbers of oocysts at the 7th days post infection,and persisted for 6 days.After dissected,the whole digestive tract mocosa were stained,oocysts were detected in small intestine.Neonatal calves were successfully infected with C. parvum and established the neonatal calves model for C. parvum.To estimate the prevalence of cryptosporidiosis in per-weaned calves in Henan Province, a total of 792 fecal samples from 8 dairy cattle farms in 7 cities in Henan Province were examined. The overall prevalence of Cryptosporidium was 21.12% (168/297) by Formalin-Ethyl acetate centrifugal precipitation method, the Sheather's sucrose flotation technique and the acid-fast staining method. Thereinto, the highest and lowest infection rate was 26.77%(34/127),10.479%(9/86)in the eight farms, respectively. The infection rate of pre-weaned( 2 week ) calves was 33.08%(44/133), and 7.27%(4/55)in pre-weaned (1 week ) calves respectively.Two species of Cryptosporidium were discovered, namely, Cryptosporidium parvum and C.anderson. Thereinto, C.parvum was the zoonotic species most commonly identified in per-weaned calves. The result of present study showed that C.parvum was of high prevalence in Henan, to estimate the public health significance of cryptosporidiosis in per-weaned calves in Henan Province.To estimate the prevalence and public health significance of cryptosporidiosis in pre-weaned calves in Henan Province. The Small-Subunit rRNA (SSU rRNA) and the 60 kDa glycoprotein (GP60) gene specific fragments of isolates from were amplifie pre-weaned calves by Nested PCR. Cryptosporidium-positive samples from 168 animals were analyzed by PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene, and were further analyzed by DNA sequencing of the PCR products. Four Cryptosporidium species C.parvum,C.bovis,C.ryanae and C.andersoni were identified. Cryptosporidium parvum and Cryptosporidium bovis was the predominant species and found in all age groups ( from 1 week to 2 months ). Sequences analysis of the gp60 gene showed that C. parvum in pre-weaned calves were all of the C. parvumâ…¡d subtype family, namedâ…¡dA19G1.The present study revealed that pre-weaned calves commonly infect Cryptosporidium parvum. Thus, pre-weaned calves may play a role in the zoonotic transmission of Cryptosporidium.
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