Identification Of MITE Tranposons And Establishment Of Molecular Marker In Microsporidia Nosema Bombycis

Nosema bombycis is a typical species of microsporidia are generally parasitic economy insects caused pebrine silkworm leading to devastated diseases which is the only legal quarantine in silkworm industry.To capture particles of this chronic disease is the important and difficult research in silkworm industry, but at home and abroad late start in N.bombycis molecular biology, that the lack of depth and breadth, but for their genome structure analysis of elements is very weak.On the other hand,use of bioinformatics and comparative genomics and other means, from the level of the related species genomic high-throughput, large-scale, high-precision studies have become hot. Transposons are a class of widely distributed mobile genetic factors in eukaryotics which lead to genetic recombination and mutation, as a type of molecular markers in species and genetic diversity has a broad application.Firstly,analysis of whole-genomic data with N.bombycis by bioinformatic in this research.Six families of MITEs (miniature inverted-repeat transposable elements)were firstly characterized in microsporidia N.bombycis, which were named NbME. The structural characteristics and the distribution of these NbME copies in N.bombycis genome were investigated. It was found that the portions of NbMEs are associated with gene sections. And on this basis, analysis of ribosomal SSUrDNA (Small subunit ribosomal DNA) on N.bombycis by sequence determination and cluster comparison, and by retrieving Chongqing genomic data and other geographic strains of SSUrDNA sequence were analyzed, showed that several copies of SSUrDNA units in N.bombycis were interrupted by NbME2, indicating that transposon insertion was the reason of causing the variability of genomic structure in N.bombycis. At the same time, in view of the Anhui, Guangxi and Chongqing in the three isolates of different geographical origin and after Spodoptera frugiperda Sf9 cells infected by corruption capacity were significantly different situation,using the NbME5 marker, MITE-AFLP technique was utilized to research the feasibility of the genetic polymorphisms among the three N.bombycis isolates,thereby, proving NbME as a potential marker to study isolate variation in N.bombycis. To further study the different isolates of N.bombycis genetic polymorphism and establish an effective basis for molecular marker system.