| Objective. With the improvement of technique in gene transfer, the study about delivery gene with capability of regulating immunologic reaction to donor heart attract more interest. Researchers want to ameliorate the ischemia-reperfusion injury and immunologic rejection after transplantation by this way. This study is to prove the feasibility of gene transfer to donor heart by ex vivo delivering intracoronari ly a reporter gene (Lac-Z gene) to donor heart at the time of heart harvesting and getting expression. Method. The model of heterotopic heart transplantation in murine was established. 12 male BALB/C mice were divided into 2 groups randomly, gene-transfer group (group 1) and control group ( group 2 ). In group 1, PSV-2001(3 -gal /liposome( DOSPER ) complex was transfered into donor heart at the time of heart harvesting, using the technique of introcoronary perfusion system; normal saline was infused In group 2. Donor hearts were harvested 3 days after operation. The transfer and expression of Lac-Z gene was detected by histochemical dyeing. Result. These donor hearts of group 1 expressed (3 -gal. The expression of (3 -gal was found in the myocardial cells in the mid-layer of ventriculus in two donor hearts, and was found in the myocardial cells under the epicardium in one donor heart. But no expression of (3 -gal was found in donor hearts of group 2. Conclusion. Ex vivo gene transfer intracoronarily to donor heart in the form of plasmid vector/1 iposome complex at the time of heart harvesting is feasible. This study provide proof to the gene therapy in heart transplantation.
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