The Study On The Origin Of The Tumor Cells In Nodular Sclerosis Hodgkin's Disease

Hodgkin's disease is a special type of lymphoid neoplasia. It is one of the major malignant neoplasms in the young of western countries, and it makes up 10 to 20 percents of all malignant lymphomas in China. Although I-ID was first described more than 160 years ago, it still represents an enigmatic disease. The etiology and the oncogenesis of I-ID remain poorly understood. HID is characterized by scattered large atypical cells residing in a complex admixture of morphologically normal cells. Hodgkin/Reed-Steinberg (H/RS) cells are regarded as malignant cells commonly. So they are focused on by many scientists in the world. Nobody in the world has detected the clonality of the background lymphocytes in HID which were thought reactive. It was known that HIRS cells only occupy a very little part in the whole tumor section, how can they make a relatively big tumor? Though many researches have been done to clarify the clonality of H/RS cell, there's no common agreement. These paradoxes make us doubt about the benign nature of the background lymphocytes and make a contrast with that of normal reactive lymphocytes. Meanwhile we also make a research about the clonality of the H/RS cells and the background lymphocytes. After that we make a contrast. The major results were as follows: 1 The proliferative activity of the lymphocytes in I-ID. Jmmunohistochemistry was applied to detect the PCNA expression in 10 cases of HD(3 cases of NSHID,7 cases of mixed cellular I-ID) and 10 cases of reactive lymph nodes. Then calculate the mean proliferative fraction in HID and reactive lymph node. The mean proliferative fraction of the background lymphocytes in HID is(18.78 ?7.1)%, whereas that of lymphocytes in active lymph nodes is(7.97 ?4.6)%. The proliferative fraction is higher significantly in background lymphocytes in I-ID than in active lymph nodes after the x 2 Test (x 2=503.36,P<0.00 1). 2.The clonality detection of Lacunar cells and lymphocytes in nodular sclerosis Hodgkin's disease. The 8 sections without coverslip of I-ID were stained with heamatoxylin-eosin. The I-IRS cells were microdissected first then the background lymphocytes next to them were microdissected. These cells were harvested and digested for PCR. One group was used for rearranged LgH gene PCR, the other was used for rearranged ICR ~ gene PCR. The lymphocytes in 6 of 8 cases of NSF-ID showed a monoclonal pattern of single discrete bands within the predicted size range(80-120 base pairs). All Lacunar cells in 8 cases of NSI-ID showed a polyclonal pattern of smear near the 100 base pairs range. The lymphocytes in 2 of 8 cases of NSF-ID showed a rearranged pattern of single discrete bands within the predicted size range (near 240 base pairs). None of Lacunar cells showed rearranged pattern of TCR v gene.