| Present laboratory diagnoses about nonA-E hepatitis still can not identify the etiology of many hepatitis patients. In Dec of 1997, Nishizawa successfully obtained 500 bp clone (N22) from a non A-non E posttransfusion hepatitis patient by representationaldifference analysis technique (RDA),it was designated astransfusion transmitted virus (TTV). TTV widely existed all over the world .It was not only prevalence in non A-non E hepatitis ,but also in A-E hepatitis , hemodialysis patients,haemophilia patients , intravenous drug users , normal populations, blood donors. Its genotypes had frequently variant .But genotypes, etioIogy, prevalence of TTV are still unclear.In 2000, Rordalisi submitted the SEN Virus (SENV) full-length nucleotide sequence on GeneBank. In the same year, Umemura also reported that SENV was related to posttransfusion hepatitis .Later, Tanaka confirmed some homology between the nudeotide and amino add sequence of SENV and TTV. He suggested SENV belong to TTV family and its nudeotide have highly divergent. Bowden and Allain considered SEN-V maybe a major cause of non-A-E hepatitis.But up to now ,based on limited available data to researchers , there were no powerful evidence. China sees no reports about SENV yet.In order to realize the dinical effect after infecting SENV , to investigate the diversity of SENV nudeotide and supply a theory foundation for immunodiagnosis and prevention of non A - non E hepatitis. We detected 495serum samples (44 non A- non E hepatitis patients ,150 A-E hepatitis patients, 21 elevated ALT infants , 86 hemodialysis patients, 35 intravenous drug users , 51 normal populations , 108 blood donors).We studied prevalence of SENV in our subjects by nested PCR. The control was an amplied fragment of TTV prototype. Then the procedures were purification, ligation, transformation of PCR positive fragment, obtainment recombinant Plasmid and DNA sequence analysis.We discovered Positive TTV PCR fragment was about 196 bp that the same as the expected. Positive SENV PCR product had two kinds of fragments, 360 bp and longer than 360 bp ones . The prevalence of SENV and TTV in different populations:1.The detection rate of TTV and SENV in 495 samples was 7.5%, 13.9% respectively.t2.The detection rate of TTV in all patients of this study ( non A-non E hepatitis patients, A-E hepatitis patients, elevated ALT infants , hemodialysis patients, intravenous drugusers ) and health people( blood donors , normal populations ) was 15.8% and 10.1% respectively, there was no significant (P>0.05). The detection rate of SENV was 11.0% , 0.0% respectively, there was significant in statistic.3. The detection rate of TTV and SENV in health people of this study was 0.0% and 10.1% respectively, there was significant.4. The detection rate of TTV and SENV in all patients of this study was 11.0% and 15.8% respectively , the simultaneous positive rate was 3.8% ,there was significant.5. The detection rate of SENV in all elevated ALT patients of this study(non A-non E hepatitis patients , A-E hepatitis patients, elevated ALT infants) and in all ALT normalpatients (hemodialysis patients, intravenous drug?users )was 14.0% and 5.8% ,respectively, there was significant. The detection rate of TTV in all ALT increment patients of this study(non A-non E hepatitis patients, A-Ehepatitis patients, ALT increment infants) and in all ALTnormal patients (hemodialysis patients, intravenous drug users )was 17.7% and 12A% respectively, there was no significant.Then PCR positve products induding TTV fragment, short and long SENV fragments were cloned in pGEM-T-Easy vector ,named respectively as pGEM-TTV, pGEM -SENVL, pGEM -SENVS.We selected the identified recombinant plasmid for DNA sequence analysis .The data was dealt with DNA Tools 5.1 and DNAStar software. The nudeotide sequence homology was 97A% between TTV PCRpositive fragment and TTV prototype(AB008394),87.7% between pGEM -SENVL(1) and SENV(AX025667), 76.1% between pGEM -S...
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