| A mouse model of photochemical induced acute cerebral infarction was established by using the orthogonal test with 5 factors and 4 levels to study the best experimental conditions. The results showed the best experiment conditions :exposure time was 5 minuts,exposure rate 1 ,dosage of rose bengal 75mg/kg,dosage of evans blue lOOmg/kg,and the time put to death 4 hours. The verification of result proved that the experiment conditions of the model were correct. This mouse model of photochemical induced acute cerebral infarction was used to select medicines which had protective effects on cerebral ischemia from Chinese herb components SSY-B2, EBY, LL-1,Tau and Sheng-Xian-Tang decoction. We fonnd that low dose of SSY-B2 and Tau reduced the evans blue抯 seepage and have protective effect on mouse model of photochemical induced acute cerebral infarction. We used rat model of middle cerebral artory occlusion to study protective effects of SSY-B2, Tau and Sheng-Xian-Tang decoction and the mechanisms from the aspects of symptoms, and the intracellular calcium ions of hippocampus and nitric oxide synthase of cortex. Middle dose of Tau and high dose of Sheng-Xian-Tang decoction reduced the increase of intracellular calcium ions of hippocampus.Middle dose of SSY-B2 and low dose of Tau and middle dose of Sheng-Xian-Tang decoction prevented the reduce of the nitric oxide synthase activity of the cortex. The mouse model of photochemical induced acute cerebral infarction is a simple and fast model for selecting protective medicines from a large number of 3 candidate drugs. This study provides experimental foundation for clinical application of these medicines.
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