| Studies on genetics showed that coronary heart disease (CHD) is a multi-factors disease.Jt is caused by the interaction of genetic and enviromental factors.Many risk factors contribute to CHD such as hypertension,hyperlipidemia, smoking and diabetes have been identified by epidemiology investigations. But at the same time,CHD shows an obvious trend of congregation.Jt infered that besides enviromental factors, genetic factors play an important role in occurrence of CI-D. In a family where there is premature Cl-lID (less than 55 years old) or there are more than one Cl-ID patients ,genetic factors play a more important role. Why different people have different response to the same enviromental risk factor? Why different people have different susceptibility to CHD? These are still unknown. Until now, etiology of Cl-lID is also unclear. Recently, studies have shown that gene polymorphisms are related to CHID susceptibility. Exploration of genes susceptible to CEllO has been a hotspot of studies on CHD etiology. Hyperlipidemia is one main contributive factor to CHD. Lipoprotein lipase(LPL) plays a central role in lipid metabolism,hydrolyzing triglyceride in chylomicrons and very low density lipoproteins. Therefore mutation in LPL gene 6 may be associated with CIID.In some previous studies,DNA variants of the LPL gene are associated with changes in lipid metabolism and occurrence of CHD. Because gene polymorphisms are different with human race and geographic location, the conclusion was different from each other.There was still no last word on the relationship between LPL gene polymorphisms and hyperlipidemia and CHD. The purpose of this study was to test whether Pvu 11 and HindIII restriction fragment length polymorphisms(RFLPs) in the LPL gene were associated with hyperlipidemia and angiographic coronary artery disease and whether they were genetic susceptibility markers of CHD.PCR-RFLP analyzing methods were used in the study.We analyzed two common LPL gene polymorphic markers, Pvu II and HindJJI in 208 patients from Xi抋n area undergoing coronary angiography.We compared Pvu II and HindJJI allele frequency in case and control groups. We assessed coronary artery disease severity as the number of significantly stenosed (50% luminal obstruction) major coronary arteries at angiography and by the Gensini coronary score.Findings were as follows: 1. In the study population, Pvu II(+) and (-) allelic frequencies were 0.66 and 0.3 4,respectively. Hind III (+) and (-) allelic frequencies were 0.58 and 0 .42,respectively. 2. There was a close relationship between the Pvu II (桰? genotype and high levels of triglyceride, P<0. 01. There was also a close relationship between the Hind III (+/+) genotype and high levels of TG and low levels of HDL-C,P<0. 01. 3. There was a close relationship between Pvu II (??genotype and the presence of CHD.The incidence of CHD in PvuII (桰? genotype was much higher than P(+I+),70.8% vs42.9%,P<0. 05. There was also a close relationship 7 between HindIJI (+7+) genotype and the presence of CHD.The incidence of CHD in HindJIJ (+1+) genotype was much higher than H(-I-) group,64.3% vs37.8%, P |
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