| Objective To study the possible mechanism of the experimental hepatic fibrosis (HF) and the protective effects and its corresponding mechanism of the compound taurine (CT) composed of taurine (Tau) and branched chain amino acid (BCAA) on the lipid peroxidation in cultured hepatocellular injury and the HF in rats. Methods Lipid peroxidation in cultured hepatocellular injury and experimental HF were induced by carbon tetrachLoride (CCl4) and inhibited by CT. The function of hepatocytes and the content of MDA were determined . The pathologic alteration of liver tissue were observed and the distribution characteristics and content of collagen type â… and â…¢ (Col-â… ,-â…¢) in the liver tissue were detected by immunhistochemistry. ALT, AST, TP, AlB, Hyp, LN, HA, MDA, SOD and GSH were measured respectively. Results After the rats were injected with the CCl4, the hepatocellular degeneration, necrosis, and lipid peroxidation took place. The content of Hyp, LN, HA in liver tissue obviously increased.The connective tissue in the interstitium of liver accumulated and the false leaflets came into being. It showed that the content of Col-â… , -â…¢ in liver tissue apparently increased in immunhistochemistry. CT can reduce the activity of transaminase and the content of MDA induced by CCl4 in cultured hepatocytes. After CT was used,the hepatocellular necrosis and the lipid peroxidation obviously alleviated and the deposition of extracellular matrix (ECM) such as collagen significantly reduced. Conclusion 1. Lipid peroxidation induced by CCl4 is the initial factor that induces HF. 2.The unbalance of metabolism and the accumulation of ECM such as collagen is the primary mechanism of HF. 4.CT can resist HF and its possible mechanism is to protect the function of hepatocytes, regulate the matebolism of collagen and inhibit the accumulation of ECM such as Col-â… , -â…¢.
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