The Research Of Synthesis Of Bioactive Peptide RGD And Method Of Its Grafting On The PET Surface

Biomaterials play an important role in human disease-treatment and healing. Diseases in cardiovascular system have higher requirement about biomaterials. In the application, traditional artificial cardiovascular materials have blood coagulation, alexin-activation and other effects in different degree which result the formation of thrombus directly or indirectly, and finally lead to the failure of implantation materials or even cause complication and endanger the patients' lives. Therefore it has been an important subject in the materials study to improve the blood compatability of cardiovascular materials.RGD is the minimum ammonia serial on the ligand, which can be recognized by most of the integrins. Connect the RGD serial on the materials surface, integrin, which comes from membrane, one of the cell adhesion molecule (CAM) on the cell surface, combines with RGD on the materials surface through ligand-acceptor. It can to some extent help the cell adhesion, spreads, grow, and finally mix to layers on the materials surface. Cell layers with biological activities form on the materials surface which can promote the blood compatibility of the biomaterials.This article is the part of "Recognizable Molecular Mode on the Polymer Materials Surface Cell and Study on the mechanism of Endothelialization."which is the Nature Science Fund project. That is covalence induce artificial combine RGD on the polymer materials surface to make endothelial cells to combine with its speciality, so that more fixed endothelializate surface.TLC method was adopted to trace the reaction process and primary test the products purity on the protected ammonia. Analysis over the combined products was carried out with the method of the testing of melting point, polarimeter and IR. RGD was purified with column chromatography. TLC XPS were used to analysis theresults. Optical microscope and SEM observe the status of the endothelial cells growth.Protection over Arg, Gly, Asp were carried and in the experiment Liquid phase synthesis and purification of RGB were taken. Protecting results from Gly protected by Boc, Asp protected by Bzl, Arg by N02 were obtained in the experiment. Pure RGD was obtained in the liquid phase synthesis. Active group -COOH was connected on the materials surface by UV radiation. The endothelialization result shows that RGD serial is successfully connected on the materials surface and improve the effect of materials endothelialization.In the future the study should be focus on the quantitation of cell growth and grafting of RGD, and the grafting of peptides longer than RGD such as RGDX following the way brought forward , and the relation of grafting and endothelialization on the condition of hydrodynamics.