Effects Of Multi-glycosidorum Triptery On Production Of Cytokine By Splenocytes In A Murine Model Of Oxazolone-induced Colitis

Multi-glycosidorum triptery(MGT) is a preparation extracted from common threewingnut root which have used in clinical therapy of many autoimmune diseases.At present, the clinical preliminary observation showed that MGT have therapeutical effect on ulcerative colitis(UC). But its mechanism i s unknown.1. Effects of Multi-glycosidorum triptery on production of IFN- and IL-4 by splenocytes in oxazolone-induced murine colitisObjectives: To investigate the in vitro effect of MGT on cytokine production by splenocytes of OXZ-induced colitis of murine model. Methods: 6mg of OXZ(in 50% of ethanol)was administered into male SJL/J mié‘• intrarectally to induce colitis and mié‘• were killed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0. Ig/L or 0. Olg/L was added to the culture medium of splenocytes. Production of in the supernatant was measuredby ELISA. ResultsiProduction of IFN- Y was suppressed by both 0. Olg/L and 0. Ig/L of MGT (1. 24 ?0. 13ng/L ?0. 97 ?0. 26ng/L-*0. 87?. 18ng/L, P<0. 02) in a dose dependent pattern. In OXZ-induced colitis group, the basic level of IFN- v was significantly lower than that of normal control group(0. 65 ?. 08ng/L vs 1.24 + 0. 13ng/L, P<0. 01),; but IL-4 production was signif icantly increased (7. 83 + 0. 69ng/L vs 5.65 + 0.48ng/L, P<0.01).In both groups, MGT suppressed IL-4 production in a dose dependent manner (normal control group: 5. 65 + 0. 48ng/L ?4. 97 + 0. 38ng/L ?3. 98 + 0. 32ng/L, P<0. 01; OXZ group: 7. 83 + 0. 69ng/L-7. 07 ?. 47ng/L 6. 35 0. 48ng/L P<0.01). Conclusion:OXZ-induced IL-4 overproduction by splenocytes was significently suppressed by MGT in a dose dependent manner; the effect of MGT on UC might be related with the suppression of Th2 type mediated immunological response of splenocytes.2. Multi-glycosidorum triptery suppressed overproduction of IFN- Y and further inhibited IL-4 production by splenocytes in nicotine-pretreated oxazolone-induced murinecolitisObjectives : To investigate the in vitro effect of Multi-glycosidorum Triptery (MGT) on cytokine production bysplenocytes of nicotine-pretreated oxazolone (OXZ)-induced colitis of murine model. Methods: Male SJL/J mice weredivided into OXZ group and nicotine pretreated group(NP group),6mg of OXZ (in 50% of ethanol) was administered intrarectally to induce colitis and mié‘• were killed 3 days later. For nicotine group, before OXZ was administered, 0. 5mg/kg /day of nicotine was injected subcutaneously for 3 weeks before the mié‘• being killed. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin with MGT of 0. Ig/L or 0. Olg/L was added to the culture medium of splenocytes, blank samples were as control. Production of IFN- y and IL-4 in the supernatant was measured by ELISA. Results: Compared with OXZ group, significantly increased IFN-Y was noticed(0. 65 ?. 08ng/L vs 2. 95 +0. 64ng/L) in NP group; MTG signif icantly inhibited nicotine stimulated overproduction of IFN~r in a dose independent manner (2. 95 + 0. 65ng/L?1. 448 + 0. 28ng/L?0. 553 ?. 07ng/L, P<0. 01). IL-4 production .in NP group was lower than that of OXZ group (6.95 + 0. 29ng/L vs 7.83 + 0. 69ng/L, P<0. 05) and was further decreased by MTG in a dose dependent manner (6. 95 + 0. 29ng/L ?. 90+ 0. 65ng/L-* 5.48 + 0.44ng/L, P<0. 05). Conclusion: MTG not only suppressed the IFN-Yoverproduction stimulated by nicotine and also had a cooperative effect with nicotine in terms of inhibition of IL-4 production . These _results suggested that MGT might counteract the deteriorating effect of nicotine on Thldominant response(IFN-y ) and have a coordinate effect withnicotine for suppression of Th2 dominant response(IL-4).