| INTRODUCTION: The gene mutation all play a role in phases of protein synthesis, thus more and more reserchers concern about the relationship of eukaryotic translation initiation factors(EIF) and cancer, especialy elFl, which the full length cDNA was not cloned at present and the reserch of its structure and function was limitied. Liu et al [1] clone a new gene from HepG2 cells by suppression subtractive hybridization and RACE PCR. They call it C2 and make the antibodies to C2. The results show a full length cDNA of C2 clone exactly 1.35 kb in length, which contains an open reading frame of 113 amino acids, corresponding to a protein of 13KDa. The deduced amino acid sequence of C2 shows 100% homology to the partial sequence of eukaryotic initiation factor elFl and high homology with human homolog of the yeast translation initiation factor, Sui 1. So C2 is a full length cDN A of human eukaryotic initiation factor elF 1. It is possible to study its function because human eukaryotic initiation factor elFl is cloned. In addition, Liu et al do further study of C2 function. They found that C2 can inhibited cell growth and the suppression of C2 by hepatitis B virus X-6-antigen(HBX) which contributes to hepatocellular carcinoma may contribute to the development of hepatocellular carcinoma(HCC).AIM AND METHODS: The aim of this study is to further analyze the distribution and significance of C2 and C2mRNA in human digestive tissues by immunohistochemistry (IHC) and in situ hybridization (ISH) and Western blottechniques. To study the expression and its significance of C2 gene in digestivesystem neoplasms tissueRESULTS1. Expression of C2 protein and C2mRNA in HCC tissue. In 26 samples of hepatocarcinoma and pericancerous tissue,the positive rate of C2mRNA were 23.1% and 76.9%, respectively. In 60 samples of hepatocarcinoma and 42 samples of pericancerous tissue, the positive rate of C2 protein were 27.3% and 83.3%, respectively. In 27 samples of liver cirrhosis,the positive rate of C2 protein were 77.8%. Statistical analysis suggested that the positive rate of C2mRNA and its protein were all significantly higher in pericancerous tissue than that in hepatocarcinoma (P (0.001) . C2 protein was significantly correlated with patient's age, HBsAg and AFP (P (0.05) , but C2 protein was not found to correlat with different pathological grading,tumor size, lymph node metastases. Result of Western blot method was consistent with that of immunohistochemical method.2. Expression of HBXAg in HCC tissue. In 60 samples of HCC, 10 samples and 7 samples were positive for C2 protein in 49 samples and 11 samples of HCC that HBXAg were positive and negative, respectively. Statistical analysis suggested that the positive rate of C2 protein were all significantly lower in HBXAg(+) tissue-7-that in HBXAg(-) tissue (P (0.05) .3. Expression of C2 protein in gastric cancer tissue. In 58 samples of gastric concern tissue and its 44 samples of pericancerous tissue, the positive rate of C2 protein were 41.4% and 77.3%, respectively. Statistical analysis suggested that the positive rate of C2 protein were all significantly higher in pericancerous tissue than that in gastric cancer tissue (P (0.05) . C2 protein was significantly correlated with different pathological grading, tumor size, the depth of invasion (P (0.05) .4. Expression of C2 protein in colorn cancer tissue. In 31 samples of colorn cancer tissue and its 23 samples of pericancerous tissue, the positive rate of C2 protein were 41.9% and 82.6%, respectively. Statistical analysis suggested that the positive rate of C2 protein were all significantly higher in pericancerous tissue than that in colorn cancer tissue (P (0.05 ) . C2 protein was significantly correlated with the depth of invasion and lymph node metastases (P <0.05) .5. Expression of C2 protein in other tumor tissues. In 23 samples of rectal cancer tissue and its 15 samples of pericancerous tissue, the positive rate of C2 protein were 43.5% and 73.3%, respec... |
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