| Bankground: Proliferative vitreoretinopathy (PVR) is an intraocular wound healing response involved in several lines of leukocytes and a number of cytokines. Retinal pigment epithelium (RPE) is the major proliferate cell and the migration of RPE is an initial step in the development of PVR. Surgical repair of PVR remains complex and time consuming, that has an important clinic signification for prevention and cure of PVR to discover the effective assistant durg to inhibit viability of RPE.Aim: This experiment aims (1) to investigate the effects of fetal bovine serum (FBS), platelet-derived growth factor(PDGF) or interleukin-lp(IL-lp) on the proliferation of cultured human RPE cells in vitro, and to test if suramin can inhibit this effect. (2) to investigate the effects of FBS, PDGF and IL-lp on the migration of RPE and the action of suramin on this effect.Methods: (1) HRPE were cultured with 10%FBS, PDGF (lOug/L) or IL ?IpClOfig/L), and with additional suramin(l.5, 15, 150 or200mg/L) for 1, 2, 3, 4 and 5 day. The effect on the proliferation of RPE cells was examined by tetrazolium colormetric assay (MTT assay) for cellular growth and survival. The proliferation viability of cells was measured by using cell division index and AgNORs staining in the supernatant of cultured RPE(3d). (2) An in vitro wound healing model was used in which a small area of a confluent monolayer of HRPE cells was denuded with a razor blade, the cultures were subsequently incubated with 10% FBS, PDGFC lOug/L) or IL- lp( 10ng/L). Suramin (1.5, 15 orl50mg/L) were added to the cultures to test its inhibition effects on the FBS, PDGF and IL-lp-induce migration of RPE cells. After 20-hr incubation, migration was measured as the number of cells that had entered the denuded area.Results: (1) The results of MTT assay showed 10%FBS, PDGF(10ug/L ) or IL ?1P (1 Oug/L) significantly stimulated the proliferation ofRPE in a time-dependent manner. At third day, compare to the blank controlwas 159%, 127% and 119%. Suramin inhibited this effect significantly (P <50.07) .The inhibitory percent of suramin 150mg/L was 51%(FBS), 26%(PDGF) and 18%(IL-1P). The cell division index and AgNORs staining were also significantly increased (P <0.07) . The 10%FBS, PDGF (lOug/L) orlL-ip (lOug/L) -induced proliferation was effectively inhibited by suramin in a dose-dependent manner,but the form of cells was uninfluenced at 150mg/L. At 250mg/L,RPE appare toxicity.(2) 10%FBS, PDGF (lOug/L) or IL~lp (lOug/L) stimulated HRPE cells migration with 437%, 382% and 285%. This effect could be inhibited by suramin in a dose-dependent manner (P < 0.07). The inhibitory percent of suramin 150mg/L was 51%(FBS), 42%(PDGF) and46%(IL-lp).Conclusions: (1) The serum, PDGF or IL梚p can accelerate the proliferation of RPE and suramin can inhibit the effects in a dose-dependent manner. (2) The serum, PDGF or IL?1P can induce the migration of RPE and suramin can inhibit the effect. (3) The serum, PDGF or IL梚p-induce migration of RPE were more effective than them-induce proliferation of RPE. (4) The inhibition effect of suramin on FBS was stronger than it on PDGF and IL-lp. The results of experiment showed that suramin was not the special inhibitor of certain cytokines, but was the synthesis effect inhibitor of multi-cytokines. Suramin will possibly become a safe and effective drug to prevent and treat PVR.
|
PDF Full Text Request