| Glioma is a kind of common brain tumour whose prognosis is not good although complex therapy such as operation, radiation, chemistry and gene threrapy is taken. Complex therapy is a progress direction of tumour therapy. Immunological and biological therapy, as a important compositive partition of complex therapy, has been regarded as great importance in medical science field recently, especially antibody-mediated immunological therapy has become one of hot spots of tumour therapy research at present. The key of antibody-mediated immunological therapy is strong specificity of target antigen, low immunogenicity, high affinity of antibody and so on. In recent years, chimeric antibody, humanized antibody, small molecular antibody, double-function antibody appeared one after another with the progress of genetic engineering antibody research. Definite therapeutic efficacy was gotten with chemical therapy medicine , toxin and radionucide attached byall of these antibodies, which powerful facilitated the research of brain glioma antibody.Brain glioma antibodies are antibodies from rats reconstructed by genetic engineering at present. Immunogenicity of rats and low affinity are the main problem in brain glioma antibody directed-therapy, so it is urgent to get humanized brain glioma antibody. But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody. We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library.In this study, blood serum in 52 glioma patients was isolated and purified, then all the glioma samples of 52 patients and malignant tumours of different tissue(including gastric carcinoma, carcinoma of endometrium, mammary gland cancer, liver cancer, thyroid cancer, transverse colon cancer, pituitary tumour of 6 samples respectively) were immunohistochemistrical stained with the blood serum of patients. Results were compared. The 52 glioma patients were detected and filtered with primary cultured normal glial cells, glioma cells and glioma cell line by enzyme linked immunosorbent assay. So the existence of brain glioma antibody in the blood of glioma patient was proved. This will be helpful that immunological mechanism of human body against brain glioma is recognized and clarified.Results indicated: 1. Positive results were gained from 3 samples in all 52 samples of glioma patients after staining with isolated and purifiedantibody. No positive result was gained from other different tissue sample. 2. Positive number was increased after staining 49 samples with the antibodies which had positive results(7 samples). 3. Positive results had significant difference between the low malignant group( I - II grade) and the high malignant group(III-IVgrade) by analyzing the positive samples. Positive results only could be seen in the low malignant group. 4. Results from enzyme linked immunosorbent assay is similar to but more than the results from immunohistochemistry.This study suggests that there is brain glioma antibody in the blood of partial glioma patient and the number of these patients is small. The antibody mainly occurs in the body of the low malignant glioma patient. Immunological characteristics of brain glioma of human body can be clarified by detecting brain glioma antibody. So the base of constructing the phage antibody library can be established and new idea can be afforded for immunological therapy of brain glioma.
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