Synthesis Of Ethyl Ferulate And Study On Its Pharmacological

Ethyl ferulate was synthesized with ferulic acid as starting material and its synthetic technics was studied. The activies of ethyl ferulate were investigated in comparison with those of ferulic acid.Section 1 Synthesis of ethyl ferulate.AIM: To esterify ferulic acid and synthesize ethyl ferulate. METHODS: Ethyl ferulate was synthesized from ferulic acid. RESULT: The melting point of ethyl ferulate was 41~42癈 which was in accord with literature. CONCLUSION: This method is feasible for the successful synthesis of ethyl ferulate Section 2 Study on the pharmacological activities of ethyl ferulate The inhibitory effect and mechanism of ethyl ferulate on plateletcongregation induced by ADPAIM: To investigate the inhibitory effect of ethyl ferulate on platelet congregate induced by ADP and platelet intracellular calcium oscillations. METHODS: To observe platelet congregate rate induced by congregater TYXN-91. Platelet intracellular calcium oscillation was observed by laserscanning .RESULTS: The inhibition rates of ethyl ferulate were 26.3 + 3.3, 33.4 ?2.4, 73.4 ?3.1 and 94.9 ?2.7% respectively at different concentrations 0.1,0.5,1.5 ,3.0 niniol-L"1 , higher than that of the group of ferulic acid. The change of API 4.6?.7 induced by ethyl ferulate was much lower than control 10.3+2.6 (n=8, P<0.01). CONCLUSION: Theinhibitory effect of ethyl ferulate on platelet congregation induced by ADP was much more than that of ferulic acid.HI . Protective effect of ethyl ferulate on acute liver injury of miceAIM: To investigate the effect of ferulic acid and ethyl ferulate on acute liver injury of mice. METHODS: (1) To divide 64 mices into 8 group .control , models , ferulic acid 25 mg-kg"1, 50 mg-kg"1,100 mg-kg"1 respectively and ethyl ferulate 25 mg-kg"1 ,50 mg-kg" ^lOOmg-kg"1 ig for 5 days respectively. In all other groups except group control , acute liver injury models of mice were made by CCL4 (156mg kg"1) ip The serum ALT , AST , SGST activities were measured. (2) To divide 48 mices into 6 group, control, models, ferulic acid 50 mg kg^and ethyl ferulate 50 mg- kg"1, 100 mg-kg"1, 200mg kg"1 ig for 5 days respectively. In all other groups except control, acute liver injury models of mice were made by CCL4 (156 mg-kg"1) ip and the level of liver MDA and SOD were measured. Liver mitochondria was observed under electronmicroscope. RESULTS: The AST, ALT, SGST levels in serum and MDA in liver tissue were significantly inhibited. It was also shown that ethyl ferulate could obviously enhanced activities of SOD. CONCLUSION: The protective effect of ethyl ferulate on mice against CCL4 acute liver injury was much better than that of ferulic acid.H . Protective effects of ethyl ferulate on injured vascular endothelialcells (EC) by H2O2.Aim: To observe protective effect of ethyl ferulate on injured vascularendothelial cells (EC) by F^Oi. METHODS: Experiments were divided into control group, model group , ferulic acid group and ethyl ferulate group. The proliferation of vascular endothelial cells was assessed by methyl thiazolyl tetrazolium (MTT) assay. RESULTS: Ethyl ferulate at concentration of0.1 umol-I/'jO.Ol umol-L"1, nmol-L"1 ,0.1 nmol-L"1 could significantly protect vascular endothelial cells from the injure by fyCh . CONCLUSION: ethyl ferulate exerted the protective effects on injured vascular endothelial cells by H2O2.