| Background & Objective : The polyraerase chain reaction (PCR) technique is one of the most advances of the molecular biology in the 21 century. It augments the specific DNA millions of multiple out of body, and has high sensitivity and specificity. With the development of the technique, more and more it was applied to the research of HBV infection, and it renewed the traditional recognition to HBV infection. The degree of hepatitis B virus (HBV) replication is assessed with HBeAg as a serological marker and HBV DNA level. However recent scientific research has presented that HBV mutations led to serum HBeAg negative and HBV DNA positive. Ongoing HBV replication in these patients is associated with a severe clinical course. Chronic hepatitis B will evolve cirrhosis carcinoma. HBV DNA concentration of the patiens with chronic hepatic diseases of HBeAg negative was detected by polymerase chain reaction (PCR), which is used to evaluate the evolution of chronic hepatic diseases, to study the pathogenicity of HBV, to assess the clinical course of HBV infection and the clinical significance of HBV serological markers, and evaluate the efficacy of the drug in the treatment of chronic hepatic diseases. To explore the correlation between the level of serum hepatitis B virus(HBV) DNA, the relevant hepatic functionindices(ALT, albumin) and the change of illness in patients of chronic hepatic diseases with HBeAg negative (including chronic hepatitis, cirrhosis and carcinoma). The relevance to HBV DNA level with the progress of chronic hepatic diseases has been evaluated and the important significance of HBV DNA level to the treatment of chronic hepatic diseases was demonstrated through the observation of HBV DNA combined with some hepatic function tests around lamivudine therapy. Methods : Ninety-one cases of chronic hepatic diseases with HBeAg negative were examined with a polymerase chain reaction (PCR) technique for the HBV DNA level detection, and the relevant analysis of the examination results was made with references to the relevant hepatic function indices and the patients' situation and the t-test was taken at the same time. Results : The average level of HBV DNA was 1. 24 E + 05 copies/ml, serum ALT and albumin were 326 IU/1, 37 g/1. The difference between HBV DNA level and the normal value was evident (P<0. 001). The HBV DNA level in 51 cases of chronic hepatitis with negative e-antigen, 30 cirrhosis and 10 hepatic carcinoma were respectively 2.OE + 05 copies/ml, 3.72E + 04 copies/ml, 2. OE + 03 copies/ml, and there was a significant difference in the comparison of two to two (P<0.05). The HBV DNA levels at the different stages (lyear, 1 ?years 5?0years 10years)of HBV infection were separately 1. 72E+05copies/mK 8. 99E+04copies/ml 7. 36E+04copies/mK 1. 26E+05copies/ml, and there was no a significant difference in the comparison of two to two(P>0.05).There was no correlation between HBV DNA level and ALT (r =-0.043, P>0. 05) ; the negative correlation withAST/ALT was obvious (r= - 0.314, p<0. 01);the positive correlation with albumin was obvious (r = 0.424, P<0.01);the positive correlation with A/G was obvious (r=0.401,p<0. 01); The levels of HBV DNA around Lamivudine administration were 3. 40E + 05 copies/ml and 4.60E + 03copies/ml, and there was a big difference between them (P<0. 001). The levels of ALT were respectively 388 IU/1 and 38 IU/1 and had important difference (P<0.001). Conclusion The patients with chronic hepatic diseases of HBeAg negative have much more HBV replication level. With the continued advance of diseases, the HBV DNA level decrease gradually. The stage of HBV infection is not associated with the HBV DNA level. The variety of high to low levels of HBV DNA is not associated with hepatic cell lesion and their degrees, but relative to the functions of hepatic anabolism and indicates illness situation toward adverse progress. The oral Lamivudine treatment can effectively restraint HBV replication, mitigate dysfunction of the liver and prevent the continued advance of diseases...
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