| Objective: To study whether β-Carotene (BC) can act as a synergist of chemotherapeutic drugs, as well as whether β-Carotene can relieve bone marrow suppression induced by chemotherapeutic drugs in animal experiments. Furthermore, to explain the mechanism of β-Carotene in a view of apoptosis. Therefore, to provide experimental evidence for clinical use of β-Carotene.Methods: There are two parts in these experiments.In the first part, we divided 80 normal TAII male mice into four groups: control group, BC group, ADM group and BC +ADM group. Then fed them with plant oil/ BC by oral every other day. A week later, injected NS/ADM into mice' tail veins. After injection, killed 5 mice of each group at 8h, 24h, 72h and 7d. Observed the change of RBC, WBC and Hb in series. Flow cytometry ( FCM), electron microscope and agarose gel electrophresis were used to analyze the apoptosis of bone marrow cells quantitatively and qualitatively.In the second part, we selected the TAII female mice transplanted with mammary cancer model to do the research. 40 mice transplanted with mammary tumor MA737 were divided into four groups: control group, BC group, CAP group and BC+CAF group. Then fed them with plant oil/BC in alternative days until the experiment was over. At the same time, treated the mice with CAF(CTX,ADM,5-Fu) or NS for 3 weeks. Observed the tumor's volume and the weight of the mice.Results: the results of the first part 1 .blood routine(1) RBC,WBC and Hb began to decrease at 72h and reached the nadir at 7d after injection of ADM.(2) The level of RBC,WBC and Hb in BC+ADM group was evidently higher than that of ADM group at the same time point (At 72h: RBC: 7.034 X 1012/L vs 5.824 X 1012 /L,P<0.01, WBC: 8.62X109 /L vs 7.34X109 /L, P<0.05, Hb:111.8g/L vs 99.4g/L,P<0.01; At7d: RBC:5.686X10l2/Lvs 3.746 X10I2/L,P<0.01, WBC:5.46X109 /Lvs3.64X109/L, P<0.01, Hb:90.2 g/L vs 70.0g/L, P<0.01).(3) BC alone could not change the RBC, WBC and Hb of the mice notably.(Compared with control group, the group of BC's RBC, WBC and Hb weren't notabledifferent at the same time point, P>0.05).2.the analysis of AI of bone marrow cells(1) The Al of bone marrow cells began to decrease at 8h after injection of ADM and reached it's nadir at the same time point. After that, it began to increase.(2) The AI of the bone marrow cells in BC +ADM group was significantly lower than that of ADM group at the same point. (8h: 16.84 % vs. 21.66%, P<0.01; 24h: 11.72% vs 14.74%, P<0.01; 72h: 6.14%vs 9.16%, P<0.01; 7d: 3.24% vs 5.24%, P<0.01) .(3) BC alone could not change the AI of bone marrow cells (Compared with control group at the same time point, the group of BC's AI weren't notable different, P>0.05). the results of the second part1. the volume of the tumor(1) After treated with CAP for 3 weeks, the volume of the tumor was significantly smaller than that of the control group.(2) The volume of the tumor in BC+CAF group was significantly smaller than that of the CAP group (515.3375 mm3 vs 1977.1250 mm3, P<0.01).(3) Feeding BC solely could not change the volume of the tumor notably (Compared with the control group : 4030.4750mm3 vs 4192.2481 mm3, P>0.05)2. the weight of the mice(1) After treated with CAP for 3 weeks, the weight of the mice was significantly lighter than that of the control group.(2) The weight of the BC+CAF group was notably weighter than that of the CAFgroup ( 18.875g vs 16.525g, P<0.01).(3) Feeding BC solely could not change the weight of the mice notably (Comparedwith the control group : 21.775g vs 21.975g, P>0.05 ).3. the AI of the tumor cells(1) After treated with CAP for 3 weeks, the AI of the tumor cells was significantly higher than that of the control group.(2) The AI of the tumor cells in BC+CAF group was notably higher than that of the CAP group (20.73% vs 15.70%, P<0.01).(3) BC alone could not change the AI of the tumor cells notably (Compared with the control group : 0.06% vs 0.05% , P>0.05).Conclusions...
|
PDF Full Text Request