| Objective:The Primary Liver cancer(PLC) is one of the highest incidence malignant tumor in our country,which is easy to metastasize in the early stage and has a high rate of recurrence after operation or intervention treatment.The death rate is the second in all kinds of malignant tumors,but the machanism of invasion and metastasis is undetermined.The emergency problem to the metastasis and recurrence of PLC in clincs is to study the sensitive,specific markers for early diagnosis and effective treatment methods. Some study showed the signal transmition and interaction of cell adhesion molecules between tumor cells,tumor cells and endothelial cells,tumor cells and matrixes are essential to invasion and metastasis of tumors.The abnormal expression of cell adhesion molecules closely related to invasion and metastasis of tumor cells.The study of the expressions of oncogenes and tumor-suppression genes as well as the products in cell adhesion family is an important way to reveal the invasion and metastasis of the liver cancer;The positive expression of AFP in the patients with PLC is only60-70% at present in our country,to study the more sensitive and specific sera markers except AFP is an important course to define early diagnosis and to evaluate the recurrence and metastasis of PLC,especially to the patients with negative expression of AFP. Intercellular adhesion molecule-1(ICAM -1) and P-selection(P-sel) belong to the Ig-super family and seletion-super family,which are very important during the tumor cell invasion and metastasis.In the present study,we detected the expression of ICAM-1 and P-sel in PLC tissues,measured the soluble ICAM-l(sICAM-l) and soluble P-selection(sP-Sel) in the sera of patients with PLC,compared with AFP, studied their relations and the relations to the development and the biological characterization of PLC,analysed the functions of sICAM-1 and sP-Sel for the early diagnosis of patients with PLC,to clarify the role of ICAM-1 and P-sel in the invasion and metastasis of PLC and anti-adhesion on molecules to PLC,to prevent recurrence and metastasis of PLC,to get an objective therapeutic evaluation for patients with PLC.Methods:Single cell suspesions,derived from 40 cases of surgically resected primary liver cancer tissues and 8 cases normal hepatic tissues,proved by pathology,were labeled with fluorescence by indirect immunofluoescence technique and studied by flow cytometry.We observed through microscopy,the protein of ICAM-1 and P-sel mostly located in the cytomembrance and cytoplasm.The apparatus type isFACS-420 made in B D Company.The results were anlysized by computer;the sera speciman of 40 cases with PLC and 20 normal sera specimen were extracted(80 sera specimen individually extracted from preoperation and 14 days postoperation),ABC-ELISA was applicated to detect the concentration of sICAM-1 and sP-sel in the sera,the full-automatic enzyme labeling apparatus type is BIO-TEK made in Bell Company.Trough the standard curve graph,we got the concentrations of sICAM-1 and sP-sel.Results:!.The positive expression of ICAM-1 protein was 100% in PLC tissues and negative expression in normal hepatic tissues. The difference was statistically significant (P<0.01).The ICAM-1 protein expression of tissues was not related to age,sex,envelope,tumor size and pathological grade(P>0.05),but had significantly statistic difference between whether had emboli and metastasized in liver or not(P<0.01).2.The positive expression of P-sel protein was 95% in PLC tissues and negative expression in normal hepatic tissues.The difference was statistically significant(P<0.001).The P-sel protein expression of tissues was not related to age,sex,envelope, tumor size and pathological grade (P>0.05),but had significantly statistic difference between whether had emboli and metastasized in liver or not(PO.Ol).3.The concentration of sICAM-1 in PLC patients was higher than the normal groups (P<0.001).The concentrationwas not related to age,sex,envelope and pathological grade(P>0.05),but had signifi...
|
PDF Full Text Request