| Deep venous thrombosis is a serious medical problem causing considerable suffering and occasional death. Thrombosis tends to occur in conjunction with surgery, fractures, Inflammatory states, immobilization, pregnancy, and the use of oral contraceptives, demonstrating circumstantial risk factors to be of importance in the pathogenesis. Recently there has been a growing interest in finding genetic polymorphisms that are associated with an increased risk of thrombosis, and a number of polymorphisms in candidate genes have been implicated. Such as FV/G1691A, FII/G20210A, and MTHFR/C677T have been discovered continuously as candidate genes of thrombosis. Not only do they undercover the possible relation of pathology and physiology between risk factors and thrombosis, but also could be used as biology signal to predict thrombosis and detect state ofsuper-coagulation.We used PCR-RFLP method to detect genotype of FV/G1691A, FII/G20210A and MTHFR/C677T in 103 DVT patients and 106 control to determine their distribution and gene frequency. We try to provide theoretical data in population genetics and thrombosis etiology, and to provide reference in prevention and treatment of venous thrombosis. Mathods: 103 DVT patients were sampled as experimental group. Diagnosis of DVT is based on intravenous angiography. 106 individuals, who came from healthy blood donors of zhengzhou were sampled as control group. The genome DNA were extracted by classic SDS-proteinase K-hydroxybenzene-chloroform methods from blood. Through PCR amplification, electrophoresis on 2% agarose gel, and EB-staining, we observed and analysed the specific segments of FV, FII, and MTHFR using lOObp DNA ladder or PUC19DNA/MspI as DNA marker in ultraviolet transilluminator. And then, the segments went on being digested with restriction endonuclear enzyme Mnll, Hindllll, and Hinfl respectively. Finally, the digested segments were analysed through 12% non-denaturation polyacryjamide gel. We coded the different genotypes in different numbers: 1 indicate wild type, 2 indicate heterozygote, 3 indicate homozygote and input SPSS 10.0 to classify and analyse.Randomly sampling 40 individuals from DVT group and control group respectively. Collected the blood serum and used ELISA method to determine ACA-IgA, ACA-IgM, and ACA-IgG according to the description. The results were expressed with binding index(BI). BI=absorbency of being checked/ absorbency of standard. Positive results were based on exceeding control mean + 2 standard deviation. Compared means between groups, we took t-test; compared the positive ratio, we took chi-square test.Using DVT as independent variable, MTHFR(T/T), ACA-IgA, ACA-IgM, ACA-IgG as dependent variable, we took Logistic regression analysis to determine who is the main risk factor of DVT.Results: The genotypes of FV/G1691A, FII/G20210A in 103 DVT patients and 106 healthy individuals were all wild types. The genotypes of MTHFR/C677T in DVT group were wild type 18(17.5%), heterozygote 57(55.3%) > homozygote 28(27.2%) separately. The genotypes of MTHFR/C677T in control group were wild type 42(39.6%)> heterozygote 48(45.3%^ homozygote 16(15.1%) separately. By chi-square test, homozygote and wild type of MTHFR/C677T between DVT group and control group had significant difference P<0.05.The level of ACA-IgM(0.52+0.35) and ACA-IgG(0.78 +0.42) in DVT group was obviously higher than that(0.31+0.22, 0.43+0.29) in control group P<0.05; The positive ratio of ACA-IgM(30%), ACA-IgG(35%) and total positive ratio (52.5%) in DVT group was obviously higher than that(5%,5% and 7.5%) in control group P<0.01.Compared wild type of ACA level between DVT group and control group, the level of IgG(0.67 + 0.32) in DVT group was obviously higher than that(0.38+0.27) in control group (P<0.01); Compared heterozygote of ACA level between them, the level of IgA, IgM, IgG between two groups had all significant difference (P<0.05); Compared homozygote, the level of IgA, IgM, IgG between two groups had not significant difference (P>0.05) .
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