The Study Of Cryopreservative Agent Of Tissue Engineered Tendon In Low Temperature

objective To investigate cryopreservative agent (CPA)to prelect tissue engineered tendon(TET) in deep low temperature. Methods The 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers(CF) and polyglycolic(PGA) were co-cultured in vitro to construct TET.lt was frozen in liquid nitrogen with four kinds of CPA for 2 months.Post-thawed quickly and transplanted into hind limbs of nude mice,and repaired the defects of achilles tendon.After 2,4,6,8,12 weeks,the morphological,histological,ultrastructure,short tandem repeat loci and immunohistochemistry examination were detected,and biomechanical strength of TET were examined.Result Tendon cell survived and could secret type I collagen after 12 weeks to transplanted into nude mice .In the group of DMSO+Raffmose+KH2O4,vacuole in mitochondrion degraded i tendon cell ranged in order,abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed.Conclusion DMSO+ Raffmose+KH2O4 could protect TET in deep low temperature.